Avicularin Inhibits Lipopolysaccharide-Induced Inflammatory Response by Suppressing ERK Phosphorylation in RAW 264.7 Macrophages

suppresAvicularin, quercetin-3-α-L-arabinofuranoside, has been reported to possess diverse pharmacological properties such as anti-inflammatory and anti-infectious effects. However, the underlying mechanism by which avicularin exerts its anti-inflammatory activity has not been clearly demonstrated....

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Main Authors:	Vo, Van Anh, Lee, Jae-Won, Chang, Ji-Eun, Kim, Ji-Young, Kim, Nam-Ho, Lee, Hee Jae, Kim, Sung-Soo, Chun, Wanjoo, Kwon, Yong-Soo
Format:	Online
Language:	English
Published:	The Korean Society of Applied Pharmacology 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3762284/

id	pubmed-3762284
recordtype	oai_dc
spelling	pubmed-37622842013-09-05 Avicularin Inhibits Lipopolysaccharide-Induced Inflammatory Response by Suppressing ERK Phosphorylation in RAW 264.7 Macrophages Vo, Van Anh Lee, Jae-Won Chang, Ji-Eun Kim, Ji-Young Kim, Nam-Ho Lee, Hee Jae Kim, Sung-Soo Chun, Wanjoo Kwon, Yong-Soo Articles suppresAvicularin, quercetin-3-α-L-arabinofuranoside, has been reported to possess diverse pharmacological properties such as anti-inflammatory and anti-infectious effects. However, the underlying mechanism by which avicularin exerts its anti-inflammatory activity has not been clearly demonstrated. This study aimed to elucidate the anti-inflammatory mechanism of avicularin in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Avicularin significantly inhibited LPS-induced excessive production of pro-inflammatory mediators such as nitric oxide (NO) and PGE2 and the protein levels of iNOS and COX-2, which are responsible for the production of NO and PGE2, respectively. Avicularin also suppressed LPS-induced overproduction of pro-inflammatory cytokine IL-1β. Furthermore, avicularin significantly suppressed LPS-induced degradation of IκB, which retains NF-κB in the cytoplasm, consequently inhibiting the transcription of pro-inflammatory genes by NF-κB in the nucleus. To understand the underlying signaling mechanism of anti-inflammatory activity of avicularin, involvement of multiple kinases was examined. Avicularin significantly attenuated LPS-induced activation of ERK signaling pathway in a concentration-dependent manner. Taken together, the present study clearly demonstrates that avicularin exhibits anti-inflammatory activity through the suppression of ERK signaling pathway in LPS-stimulated RAW 264.7 macrophage cells. The Korean Society of Applied Pharmacology 2012-11 /pmc/articles/PMC3762284/ /pubmed/24009846 http://dx.doi.org/10.4062/biomolther.2012.20.6.532 Text en Copyright ©2012, The Korean Society of Pharmaceutics
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Vo, Van Anh Lee, Jae-Won Chang, Ji-Eun Kim, Ji-Young Kim, Nam-Ho Lee, Hee Jae Kim, Sung-Soo Chun, Wanjoo Kwon, Yong-Soo
spellingShingle	Vo, Van Anh Lee, Jae-Won Chang, Ji-Eun Kim, Ji-Young Kim, Nam-Ho Lee, Hee Jae Kim, Sung-Soo Chun, Wanjoo Kwon, Yong-Soo Avicularin Inhibits Lipopolysaccharide-Induced Inflammatory Response by Suppressing ERK Phosphorylation in RAW 264.7 Macrophages
author_facet	Vo, Van Anh Lee, Jae-Won Chang, Ji-Eun Kim, Ji-Young Kim, Nam-Ho Lee, Hee Jae Kim, Sung-Soo Chun, Wanjoo Kwon, Yong-Soo
author_sort	Vo, Van Anh
title	Avicularin Inhibits Lipopolysaccharide-Induced Inflammatory Response by Suppressing ERK Phosphorylation in RAW 264.7 Macrophages
title_short	Avicularin Inhibits Lipopolysaccharide-Induced Inflammatory Response by Suppressing ERK Phosphorylation in RAW 264.7 Macrophages
title_full	Avicularin Inhibits Lipopolysaccharide-Induced Inflammatory Response by Suppressing ERK Phosphorylation in RAW 264.7 Macrophages
title_fullStr	Avicularin Inhibits Lipopolysaccharide-Induced Inflammatory Response by Suppressing ERK Phosphorylation in RAW 264.7 Macrophages
title_full_unstemmed	Avicularin Inhibits Lipopolysaccharide-Induced Inflammatory Response by Suppressing ERK Phosphorylation in RAW 264.7 Macrophages
title_sort	avicularin inhibits lipopolysaccharide-induced inflammatory response by suppressing erk phosphorylation in raw 264.7 macrophages
description	suppresAvicularin, quercetin-3-α-L-arabinofuranoside, has been reported to possess diverse pharmacological properties such as anti-inflammatory and anti-infectious effects. However, the underlying mechanism by which avicularin exerts its anti-inflammatory activity has not been clearly demonstrated. This study aimed to elucidate the anti-inflammatory mechanism of avicularin in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Avicularin significantly inhibited LPS-induced excessive production of pro-inflammatory mediators such as nitric oxide (NO) and PGE2 and the protein levels of iNOS and COX-2, which are responsible for the production of NO and PGE2, respectively. Avicularin also suppressed LPS-induced overproduction of pro-inflammatory cytokine IL-1β. Furthermore, avicularin significantly suppressed LPS-induced degradation of IκB, which retains NF-κB in the cytoplasm, consequently inhibiting the transcription of pro-inflammatory genes by NF-κB in the nucleus. To understand the underlying signaling mechanism of anti-inflammatory activity of avicularin, involvement of multiple kinases was examined. Avicularin significantly attenuated LPS-induced activation of ERK signaling pathway in a concentration-dependent manner. Taken together, the present study clearly demonstrates that avicularin exhibits anti-inflammatory activity through the suppression of ERK signaling pathway in LPS-stimulated RAW 264.7 macrophage cells.
publisher	The Korean Society of Applied Pharmacology
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3762284/
_version_	1612008181514371072

Avicularin Inhibits Lipopolysaccharide-Induced Inflammatory Response by Suppressing ERK Phosphorylation in RAW 264.7 Macrophages

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