Isolation, Identification and Characterization of an Electrogenic Microalgae Strain
Extracellular electron transfer involving microbes is important as it closely reflects the ability of cells to communicate with the environment. However, there are few reports on electron transfer mechanisms of pure microalgae and a lack of any model alga to study the transfer processes. In the pres...
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| Format: | Online |
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Public Library of Science
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760914/ |
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pubmed-3760914 |
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oai_dc |
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pubmed-37609142013-09-09 Isolation, Identification and Characterization of an Electrogenic Microalgae Strain Wu, Yicheng Guan, Kai Wang, Zejie Xu, Bing Zhao, Feng Research Article Extracellular electron transfer involving microbes is important as it closely reflects the ability of cells to communicate with the environment. However, there are few reports on electron transfer mechanisms of pure microalgae and a lack of any model alga to study the transfer processes. In the present study, nine green microalgae species were isolated from wastewater and characterized in terms of their ability to transfer electrons between cells and an electrode. One species showed direct electron transfer via membrane-associated proteins and indirect electron transfer via secreted oxygen. The microalga was identified as Desmodesmus sp. based on phylogenetic analysis and electron microscopy. Electrochemical tests demonstrated that Desmodesmus sp. was able to act as a cathodic microorganism. Stable current densities of −0.24, 35.54 and 170 mA m−2 were achieved at potentials of +0.2, −0.2 and −0.4 V, respectively, under illumination. Dissolved oxygen concentration measurement showed gradients within the microalgae biofilm: 18.3 mg L−1 in light decreasing to 4.29 mg L−1 in the dark. This study diversified the exoelectrogen library and provided a potential model microalga to explore the associated mechanism of extracellular electron transfer. Public Library of Science 2013-09-03 /pmc/articles/PMC3760914/ /pubmed/24019922 http://dx.doi.org/10.1371/journal.pone.0073442 Text en © 2013 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Wu, Yicheng Guan, Kai Wang, Zejie Xu, Bing Zhao, Feng |
| spellingShingle |
Wu, Yicheng Guan, Kai Wang, Zejie Xu, Bing Zhao, Feng Isolation, Identification and Characterization of an Electrogenic Microalgae Strain |
| author_facet |
Wu, Yicheng Guan, Kai Wang, Zejie Xu, Bing Zhao, Feng |
| author_sort |
Wu, Yicheng |
| title |
Isolation, Identification and Characterization of an Electrogenic Microalgae Strain |
| title_short |
Isolation, Identification and Characterization of an Electrogenic Microalgae Strain |
| title_full |
Isolation, Identification and Characterization of an Electrogenic Microalgae Strain |
| title_fullStr |
Isolation, Identification and Characterization of an Electrogenic Microalgae Strain |
| title_full_unstemmed |
Isolation, Identification and Characterization of an Electrogenic Microalgae Strain |
| title_sort |
isolation, identification and characterization of an electrogenic microalgae strain |
| description |
Extracellular electron transfer involving microbes is important as it closely reflects the ability of cells to communicate with the environment. However, there are few reports on electron transfer mechanisms of pure microalgae and a lack of any model alga to study the transfer processes. In the present study, nine green microalgae species were isolated from wastewater and characterized in terms of their ability to transfer electrons between cells and an electrode. One species showed direct electron transfer via membrane-associated proteins and indirect electron transfer via secreted oxygen. The microalga was identified as Desmodesmus sp. based on phylogenetic analysis and electron microscopy. Electrochemical tests demonstrated that Desmodesmus sp. was able to act as a cathodic microorganism. Stable current densities of −0.24, 35.54 and 170 mA m−2 were achieved at potentials of +0.2, −0.2 and −0.4 V, respectively, under illumination. Dissolved oxygen concentration measurement showed gradients within the microalgae biofilm: 18.3 mg L−1 in light decreasing to 4.29 mg L−1 in the dark. This study diversified the exoelectrogen library and provided a potential model microalga to explore the associated mechanism of extracellular electron transfer. |
| publisher |
Public Library of Science |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760914/ |
| _version_ |
1612007998850334720 |