Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis
Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration o...
| Main Authors: | , , , , |
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| Format: | Online |
| Language: | English |
| Published: |
Co-Action Publishing
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3760643/ |
| Summary: | Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique. |
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