WNT3 Is a Biomarker Capable of Predicting the Definitive Endoderm Differentiation Potential of hESCs

Generation of functional cells from human pluripotent stem cells (PSCs) through in vitro differentiation is a promising approach for drug screening and cell therapy. However, the observed large and unavoidable variation in the differentiation potential of different human embryonic stem cell (hESC)/i...

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Main Authors:	Jiang, Wei, Zhang, Donghui, Bursac, Nenad, Zhang, Yi
Format:	Online
Language:	English
Published:	Elsevier 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757741/

id	pubmed-3757741
recordtype	oai_dc
spelling	pubmed-37577412013-09-17 WNT3 Is a Biomarker Capable of Predicting the Definitive Endoderm Differentiation Potential of hESCs Jiang, Wei Zhang, Donghui Bursac, Nenad Zhang, Yi Report Generation of functional cells from human pluripotent stem cells (PSCs) through in vitro differentiation is a promising approach for drug screening and cell therapy. However, the observed large and unavoidable variation in the differentiation potential of different human embryonic stem cell (hESC)/induced PSC (iPSC) lines makes the selection of an appropriate cell line for the differentiation of a particular cell lineage difficult. Here, we report identification of WNT3 as a biomarker capable of predicting definitive endoderm (DE) differentiation potential of hESCs. We show that the mRNA level of WNT3 in hESCs correlates with their DE differentiation efficiency. In addition, manipulations of hESCs through WNT3 knockdown or overexpression can respectively inhibit or promote DE differentiation in a WNT3 level-dependent manner. Finally, analysis of several hESC lines based on their WNT3 expression levels allowed accurate prediction of their DE differentiation potential. Collectively, our study supports the notion that WNT3 can serve as a biomarker for predicting DE differentiation potential of hESCs. Elsevier 2013-06-04 /pmc/articles/PMC3757741/ /pubmed/24052941 http://dx.doi.org/10.1016/j.stemcr.2013.03.003 Text en © 2013 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Jiang, Wei Zhang, Donghui Bursac, Nenad Zhang, Yi
spellingShingle	Jiang, Wei Zhang, Donghui Bursac, Nenad Zhang, Yi WNT3 Is a Biomarker Capable of Predicting the Definitive Endoderm Differentiation Potential of hESCs
author_facet	Jiang, Wei Zhang, Donghui Bursac, Nenad Zhang, Yi
author_sort	Jiang, Wei
title	WNT3 Is a Biomarker Capable of Predicting the Definitive Endoderm Differentiation Potential of hESCs
title_short	WNT3 Is a Biomarker Capable of Predicting the Definitive Endoderm Differentiation Potential of hESCs
title_full	WNT3 Is a Biomarker Capable of Predicting the Definitive Endoderm Differentiation Potential of hESCs
title_fullStr	WNT3 Is a Biomarker Capable of Predicting the Definitive Endoderm Differentiation Potential of hESCs
title_full_unstemmed	WNT3 Is a Biomarker Capable of Predicting the Definitive Endoderm Differentiation Potential of hESCs
title_sort	wnt3 is a biomarker capable of predicting the definitive endoderm differentiation potential of hescs
description	Generation of functional cells from human pluripotent stem cells (PSCs) through in vitro differentiation is a promising approach for drug screening and cell therapy. However, the observed large and unavoidable variation in the differentiation potential of different human embryonic stem cell (hESC)/induced PSC (iPSC) lines makes the selection of an appropriate cell line for the differentiation of a particular cell lineage difficult. Here, we report identification of WNT3 as a biomarker capable of predicting definitive endoderm (DE) differentiation potential of hESCs. We show that the mRNA level of WNT3 in hESCs correlates with their DE differentiation efficiency. In addition, manipulations of hESCs through WNT3 knockdown or overexpression can respectively inhibit or promote DE differentiation in a WNT3 level-dependent manner. Finally, analysis of several hESC lines based on their WNT3 expression levels allowed accurate prediction of their DE differentiation potential. Collectively, our study supports the notion that WNT3 can serve as a biomarker for predicting DE differentiation potential of hESCs.
publisher	Elsevier
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3757741/
_version_	1612007079781859328

WNT3 Is a Biomarker Capable of Predicting the Definitive Endoderm Differentiation Potential of hESCs

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