Cytotoxicity evaluation of carbon-encapsulated iron nanoparticles in melanoma cells and dermal fibroblasts

Carbon-encapsulated iron nanoparticles (CEINs) are emerging as promising biomedical tools due to their unique physicochemical properties. In this study, the cytotoxic effect of CEINs (the mean diameter distribution ranges 46–56 nm) has been explored by MTT, LDH leakage, Calcein-AM/propidium iodide (...

Full description

Bibliographic Details
Main Authors:	Grudzinski, Ireneusz P., Bystrzejewski, Michal, Cywinska, Monika A., Kosmider, Anita, Poplawska, Magdalena, Cieszanowski, Andrzej, Ostrowska, Agnieszka
Format:	Online
Language:	English
Published:	Springer Netherlands 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751228/

id	pubmed-3751228
recordtype	oai_dc
spelling	pubmed-37512282013-08-27 Cytotoxicity evaluation of carbon-encapsulated iron nanoparticles in melanoma cells and dermal fibroblasts Grudzinski, Ireneusz P. Bystrzejewski, Michal Cywinska, Monika A. Kosmider, Anita Poplawska, Magdalena Cieszanowski, Andrzej Ostrowska, Agnieszka Research Paper Carbon-encapsulated iron nanoparticles (CEINs) are emerging as promising biomedical tools due to their unique physicochemical properties. In this study, the cytotoxic effect of CEINs (the mean diameter distribution ranges 46–56 nm) has been explored by MTT, LDH leakage, Calcein-AM/propidium iodide (PI) and Annexin V-FITC/PI assays in human melanoma (HTB-140), mouse melanoma (B16-F10) cells, and human dermal fibroblasts (HDFs). The results demonstrated that CEINs produce mitochondrial and cell membrane cytotoxicities in a dose (0.0001–100 μg/ml)-dependent manner. Moreover, the studies elucidated some differences in cytotoxic effects between CEINs used as raw and purified materials composing of the carbon surface with acidic groups. Experiments showed that HTB-140 cells are more sensitive to prone early apoptotic events due to raw CEINs as compared to B16-F10 or HDF cells, respectively. Taken together, these results suggest that the amount of CEINs administered to cells and the composition of CEINs containing different amounts of iron as well as the carbon surface modification type is critical determinant of cytotoxic responses in both normal and cancer (melanoma) cells. Springer Netherlands 2013-07-24 2013 /pmc/articles/PMC3751228/ /pubmed/23990753 http://dx.doi.org/10.1007/s11051-013-1835-7 Text en © The Author(s) 2013 Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Grudzinski, Ireneusz P. Bystrzejewski, Michal Cywinska, Monika A. Kosmider, Anita Poplawska, Magdalena Cieszanowski, Andrzej Ostrowska, Agnieszka
spellingShingle	Grudzinski, Ireneusz P. Bystrzejewski, Michal Cywinska, Monika A. Kosmider, Anita Poplawska, Magdalena Cieszanowski, Andrzej Ostrowska, Agnieszka Cytotoxicity evaluation of carbon-encapsulated iron nanoparticles in melanoma cells and dermal fibroblasts
author_facet	Grudzinski, Ireneusz P. Bystrzejewski, Michal Cywinska, Monika A. Kosmider, Anita Poplawska, Magdalena Cieszanowski, Andrzej Ostrowska, Agnieszka
author_sort	Grudzinski, Ireneusz P.
title	Cytotoxicity evaluation of carbon-encapsulated iron nanoparticles in melanoma cells and dermal fibroblasts
title_short	Cytotoxicity evaluation of carbon-encapsulated iron nanoparticles in melanoma cells and dermal fibroblasts
title_full	Cytotoxicity evaluation of carbon-encapsulated iron nanoparticles in melanoma cells and dermal fibroblasts
title_fullStr	Cytotoxicity evaluation of carbon-encapsulated iron nanoparticles in melanoma cells and dermal fibroblasts
title_full_unstemmed	Cytotoxicity evaluation of carbon-encapsulated iron nanoparticles in melanoma cells and dermal fibroblasts
title_sort	cytotoxicity evaluation of carbon-encapsulated iron nanoparticles in melanoma cells and dermal fibroblasts
description	Carbon-encapsulated iron nanoparticles (CEINs) are emerging as promising biomedical tools due to their unique physicochemical properties. In this study, the cytotoxic effect of CEINs (the mean diameter distribution ranges 46–56 nm) has been explored by MTT, LDH leakage, Calcein-AM/propidium iodide (PI) and Annexin V-FITC/PI assays in human melanoma (HTB-140), mouse melanoma (B16-F10) cells, and human dermal fibroblasts (HDFs). The results demonstrated that CEINs produce mitochondrial and cell membrane cytotoxicities in a dose (0.0001–100 μg/ml)-dependent manner. Moreover, the studies elucidated some differences in cytotoxic effects between CEINs used as raw and purified materials composing of the carbon surface with acidic groups. Experiments showed that HTB-140 cells are more sensitive to prone early apoptotic events due to raw CEINs as compared to B16-F10 or HDF cells, respectively. Taken together, these results suggest that the amount of CEINs administered to cells and the composition of CEINs containing different amounts of iron as well as the carbon surface modification type is critical determinant of cytotoxic responses in both normal and cancer (melanoma) cells.
publisher	Springer Netherlands
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3751228/
_version_	1612005429011808256

Cytotoxicity evaluation of carbon-encapsulated iron nanoparticles in melanoma cells and dermal fibroblasts

Similar Items