Sustained Release of BMP-2 in Bioprinted Alginate for Osteogenicity in Mice and Rats

The design of bioactive three-dimensional (3D) scaffolds is a major focus in bone tissue engineering. Incorporation of growth factors into bioprinted scaffolds offers many new possibilities regarding both biological and architectural properties of the scaffolds. This study investigates whether the s...

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Main Authors:	Poldervaart, Michelle T., Wang, Huanan, van der Stok, Johan, Weinans, Harrie, Leeuwenburgh, Sander C. G., Öner, F. Cumhur, Dhert, Wouter J. A., Alblas, Jacqueline
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747086/

id	pubmed-3747086
recordtype	oai_dc
spelling	pubmed-37470862013-08-23 Sustained Release of BMP-2 in Bioprinted Alginate for Osteogenicity in Mice and Rats Poldervaart, Michelle T. Wang, Huanan van der Stok, Johan Weinans, Harrie Leeuwenburgh, Sander C. G. Öner, F. Cumhur Dhert, Wouter J. A. Alblas, Jacqueline Research Article The design of bioactive three-dimensional (3D) scaffolds is a major focus in bone tissue engineering. Incorporation of growth factors into bioprinted scaffolds offers many new possibilities regarding both biological and architectural properties of the scaffolds. This study investigates whether the sustained release of bone morphogenetic protein 2 (BMP-2) influences osteogenicity of tissue engineered bioprinted constructs. BMP-2 loaded on gelatin microparticles (GMPs) was used as a sustained release system, which was dispersed in hydrogel-based constructs and compared to direct inclusion of BMP-2 in alginate or control GMPs. The constructs were supplemented with goat multipotent stromal cells (gMSCs) and biphasic calcium phosphate to study osteogenic differentiation and bone formation respectively. BMP-2 release kinetics and bioactivity showed continuous release for three weeks coinciding with osteogenicity. Osteogenic differentiation and bone formation of bioprinted GMP containing constructs were investigated after subcutaneous implantation in mice or rats. BMP-2 significantly increased bone formation, which was not influenced by the release timing. We showed that 3D printing of controlled release particles is feasible and that the released BMP-2 directs osteogenic differentiation in vitro and in vivo. Public Library of Science 2013-08-19 /pmc/articles/PMC3747086/ /pubmed/23977328 http://dx.doi.org/10.1371/journal.pone.0072610 Text en © 2013 Poldervaart et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Poldervaart, Michelle T. Wang, Huanan van der Stok, Johan Weinans, Harrie Leeuwenburgh, Sander C. G. Öner, F. Cumhur Dhert, Wouter J. A. Alblas, Jacqueline
spellingShingle	Poldervaart, Michelle T. Wang, Huanan van der Stok, Johan Weinans, Harrie Leeuwenburgh, Sander C. G. Öner, F. Cumhur Dhert, Wouter J. A. Alblas, Jacqueline Sustained Release of BMP-2 in Bioprinted Alginate for Osteogenicity in Mice and Rats
author_facet	Poldervaart, Michelle T. Wang, Huanan van der Stok, Johan Weinans, Harrie Leeuwenburgh, Sander C. G. Öner, F. Cumhur Dhert, Wouter J. A. Alblas, Jacqueline
author_sort	Poldervaart, Michelle T.
title	Sustained Release of BMP-2 in Bioprinted Alginate for Osteogenicity in Mice and Rats
title_short	Sustained Release of BMP-2 in Bioprinted Alginate for Osteogenicity in Mice and Rats
title_full	Sustained Release of BMP-2 in Bioprinted Alginate for Osteogenicity in Mice and Rats
title_fullStr	Sustained Release of BMP-2 in Bioprinted Alginate for Osteogenicity in Mice and Rats
title_full_unstemmed	Sustained Release of BMP-2 in Bioprinted Alginate for Osteogenicity in Mice and Rats
title_sort	sustained release of bmp-2 in bioprinted alginate for osteogenicity in mice and rats
description	The design of bioactive three-dimensional (3D) scaffolds is a major focus in bone tissue engineering. Incorporation of growth factors into bioprinted scaffolds offers many new possibilities regarding both biological and architectural properties of the scaffolds. This study investigates whether the sustained release of bone morphogenetic protein 2 (BMP-2) influences osteogenicity of tissue engineered bioprinted constructs. BMP-2 loaded on gelatin microparticles (GMPs) was used as a sustained release system, which was dispersed in hydrogel-based constructs and compared to direct inclusion of BMP-2 in alginate or control GMPs. The constructs were supplemented with goat multipotent stromal cells (gMSCs) and biphasic calcium phosphate to study osteogenic differentiation and bone formation respectively. BMP-2 release kinetics and bioactivity showed continuous release for three weeks coinciding with osteogenicity. Osteogenic differentiation and bone formation of bioprinted GMP containing constructs were investigated after subcutaneous implantation in mice or rats. BMP-2 significantly increased bone formation, which was not influenced by the release timing. We showed that 3D printing of controlled release particles is feasible and that the released BMP-2 directs osteogenic differentiation in vitro and in vivo.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3747086/
_version_	1612003985321885696

Sustained Release of BMP-2 in Bioprinted Alginate for Osteogenicity in Mice and Rats

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