EMMPRIN expression positively correlates with WHO grades of astrocytomas and meningiomas

High-grade primary brain tumors possessed poor outcome due to invasiveness. Extracellular matrix metalloproteinase inducer (EMMPRIN) stimulates peri-tumoral fibroblasts to secrete matrix metalloproteinase and promote invasiveness. This study hypothesized that high-grade brain tumors overexpress EMMP...

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Main Authors:	Tsai, Wen-Chiuan, Chen, Ying, Huang, Li-Chun, Lee, Herng-Sheng, Ma, Hsin-I, Huang, Shih-Ming, Sytwu, Huey-Kang, Hueng, Dueng-Yuan
Format:	Online
Language:	English
Published:	Springer US 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3744650/

id	pubmed-3744650
recordtype	oai_dc
spelling	pubmed-37446502013-08-16 EMMPRIN expression positively correlates with WHO grades of astrocytomas and meningiomas Tsai, Wen-Chiuan Chen, Ying Huang, Li-Chun Lee, Herng-Sheng Ma, Hsin-I Huang, Shih-Ming Sytwu, Huey-Kang Hueng, Dueng-Yuan Laboratory Investigation High-grade primary brain tumors possessed poor outcome due to invasiveness. Extracellular matrix metalloproteinase inducer (EMMPRIN) stimulates peri-tumoral fibroblasts to secrete matrix metalloproteinase and promote invasiveness. This study hypothesized that high-grade brain tumors overexpress EMMPRIN. Analyzing the public delinked database from the Gene Expression Omnibus profile, the results showed that the EMMPRIN mRNA level was higher in WHO grade IV (n = 81) than in grade III (n = 19, p < 0.0005) astrocytomas and non-tumor brain tissue controls (n = 23, p < 0.00001). The results of tissue microarray-based immunohistochemical (IHC) staining revealed that EMMPRIN levels positively correlated with WHO grades for astrocytomas (p = 0.008) and meningiomas (p = 0.048). EMMPRIN mRNA levels in conventional glioma cell lines (n = 36) was not less than those in glioma primary culture cells (n = 27) and glioblastoma stem-like cells (n = 12). The GBM8401, U87MG, and LN229 human glioma cell lines also overexpressed EMMPRIN. Hematoxylin and eosin, IHC, and immunofluorescence staining of xenografts confirmed that high-grade brain tumors overexpressed EMMPRIN. Lastly, Kaplan–Meier analysis revealed poorer survival in WHO grade IV (n = 56) than in grade III astrocytomas (n = 21, by log-rank test; p = 0.0001, 95 % CI: 1.842–3.053). However, in high-grade astrocytomas, there was no difference in survival between high and low EMMPRIN mRNA levels. Thus, this study identified that high-grade brain tumors overexpress EMMPRIN, which positively correlates with WHO grades in human astrocytomas and meningiomas, and suggests that EMMPRIN may be a therapeutic target of brain tumor. Springer US 2013-07-02 2013 /pmc/articles/PMC3744650/ /pubmed/23817808 http://dx.doi.org/10.1007/s11060-013-1184-5 Text en © The Author(s) 2013 Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Tsai, Wen-Chiuan Chen, Ying Huang, Li-Chun Lee, Herng-Sheng Ma, Hsin-I Huang, Shih-Ming Sytwu, Huey-Kang Hueng, Dueng-Yuan
spellingShingle	Tsai, Wen-Chiuan Chen, Ying Huang, Li-Chun Lee, Herng-Sheng Ma, Hsin-I Huang, Shih-Ming Sytwu, Huey-Kang Hueng, Dueng-Yuan EMMPRIN expression positively correlates with WHO grades of astrocytomas and meningiomas
author_facet	Tsai, Wen-Chiuan Chen, Ying Huang, Li-Chun Lee, Herng-Sheng Ma, Hsin-I Huang, Shih-Ming Sytwu, Huey-Kang Hueng, Dueng-Yuan
author_sort	Tsai, Wen-Chiuan
title	EMMPRIN expression positively correlates with WHO grades of astrocytomas and meningiomas
title_short	EMMPRIN expression positively correlates with WHO grades of astrocytomas and meningiomas
title_full	EMMPRIN expression positively correlates with WHO grades of astrocytomas and meningiomas
title_fullStr	EMMPRIN expression positively correlates with WHO grades of astrocytomas and meningiomas
title_full_unstemmed	EMMPRIN expression positively correlates with WHO grades of astrocytomas and meningiomas
title_sort	emmprin expression positively correlates with who grades of astrocytomas and meningiomas
description	High-grade primary brain tumors possessed poor outcome due to invasiveness. Extracellular matrix metalloproteinase inducer (EMMPRIN) stimulates peri-tumoral fibroblasts to secrete matrix metalloproteinase and promote invasiveness. This study hypothesized that high-grade brain tumors overexpress EMMPRIN. Analyzing the public delinked database from the Gene Expression Omnibus profile, the results showed that the EMMPRIN mRNA level was higher in WHO grade IV (n = 81) than in grade III (n = 19, p < 0.0005) astrocytomas and non-tumor brain tissue controls (n = 23, p < 0.00001). The results of tissue microarray-based immunohistochemical (IHC) staining revealed that EMMPRIN levels positively correlated with WHO grades for astrocytomas (p = 0.008) and meningiomas (p = 0.048). EMMPRIN mRNA levels in conventional glioma cell lines (n = 36) was not less than those in glioma primary culture cells (n = 27) and glioblastoma stem-like cells (n = 12). The GBM8401, U87MG, and LN229 human glioma cell lines also overexpressed EMMPRIN. Hematoxylin and eosin, IHC, and immunofluorescence staining of xenografts confirmed that high-grade brain tumors overexpressed EMMPRIN. Lastly, Kaplan–Meier analysis revealed poorer survival in WHO grade IV (n = 56) than in grade III astrocytomas (n = 21, by log-rank test; p = 0.0001, 95 % CI: 1.842–3.053). However, in high-grade astrocytomas, there was no difference in survival between high and low EMMPRIN mRNA levels. Thus, this study identified that high-grade brain tumors overexpress EMMPRIN, which positively correlates with WHO grades in human astrocytomas and meningiomas, and suggests that EMMPRIN may be a therapeutic target of brain tumor.
publisher	Springer US
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3744650/
_version_	1612003417358598144

EMMPRIN expression positively correlates with WHO grades of astrocytomas and meningiomas

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