Upregulation of Phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in Acquired Middle Ear Cholesteatoma Growth

Cholesteatoma is a destructive and expanding growth of keratinizing squamous epithelium in the middle ear or petrous apex. The molecular and cellular processes of the pathogenesis of acquired middle ear cholesteatoma have not been fully understood. In this study, comparative proteomic analysis was c...

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Main Authors:	Ho, Kuen Yao, Yeh, Tai Sheng, Huang, Han Hsiang, Hung, Kuo Feng, Chai, Chee Yin, Chen, Wan Tzu, Tsai, Shih Meng, Chang, Ning Chia, Chien, Chen Yu, Wang, Hsun Mo, Wu, Yu Jen
Format:	Online
Language:	English
Published:	Molecular Diversity Preservation International (MDPI) 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742253/

id	pubmed-3742253
recordtype	oai_dc
spelling	pubmed-37422532013-08-13 Upregulation of Phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in Acquired Middle Ear Cholesteatoma Growth Ho, Kuen Yao Yeh, Tai Sheng Huang, Han Hsiang Hung, Kuo Feng Chai, Chee Yin Chen, Wan Tzu Tsai, Shih Meng Chang, Ning Chia Chien, Chen Yu Wang, Hsun Mo Wu, Yu Jen Article Cholesteatoma is a destructive and expanding growth of keratinizing squamous epithelium in the middle ear or petrous apex. The molecular and cellular processes of the pathogenesis of acquired middle ear cholesteatoma have not been fully understood. In this study, comparative proteomic analysis was conducted to investigate the roles of specific proteins in the pathways regarding keratinocyte proliferation in cholesteatoma. The differential proteins were detected by comparing the two-dimension electrophoresis (2-DE) maps of the epithelial tissues of 12 attic cholesteatomas with those of retroauricular skins. There were 14 upregulated proteins in the epithelial tissues of cholesteatoma in comparison with retroauricular skin. The modulation of five crucial proteins, HSP27, PRDX2, GRP75, GRP78 and GRP94, was further determined by RT-PCR, Western blot and immunohistochemistry. Phosphorylation of HSP27 at Ser-82 was identified by mass spectroscopy. The results of this study suggested that phosphorylated HSP27 is the end expression of two potential signal-transduction pathways, and together with PRDX2, they are very likely involved in the proliferation of keratinocytes in cholesteatoma. Upregulations of GRP75, GRP78 and GRP94 in keratinocytes may be able to counter endoplasmic reticulum stress, to inhibit cell apoptosis, to prevent protein unfolding and to promote cholesteatoma growth. Molecular Diversity Preservation International (MDPI) 2013-07-11 /pmc/articles/PMC3742253/ /pubmed/23852020 http://dx.doi.org/10.3390/ijms140714439 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ho, Kuen Yao Yeh, Tai Sheng Huang, Han Hsiang Hung, Kuo Feng Chai, Chee Yin Chen, Wan Tzu Tsai, Shih Meng Chang, Ning Chia Chien, Chen Yu Wang, Hsun Mo Wu, Yu Jen
spellingShingle	Ho, Kuen Yao Yeh, Tai Sheng Huang, Han Hsiang Hung, Kuo Feng Chai, Chee Yin Chen, Wan Tzu Tsai, Shih Meng Chang, Ning Chia Chien, Chen Yu Wang, Hsun Mo Wu, Yu Jen Upregulation of Phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in Acquired Middle Ear Cholesteatoma Growth
author_facet	Ho, Kuen Yao Yeh, Tai Sheng Huang, Han Hsiang Hung, Kuo Feng Chai, Chee Yin Chen, Wan Tzu Tsai, Shih Meng Chang, Ning Chia Chien, Chen Yu Wang, Hsun Mo Wu, Yu Jen
author_sort	Ho, Kuen Yao
title	Upregulation of Phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in Acquired Middle Ear Cholesteatoma Growth
title_short	Upregulation of Phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in Acquired Middle Ear Cholesteatoma Growth
title_full	Upregulation of Phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in Acquired Middle Ear Cholesteatoma Growth
title_fullStr	Upregulation of Phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in Acquired Middle Ear Cholesteatoma Growth
title_full_unstemmed	Upregulation of Phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in Acquired Middle Ear Cholesteatoma Growth
title_sort	upregulation of phosphorylated hsp27, prdx2, grp75, grp78 and grp94 in acquired middle ear cholesteatoma growth
description	Cholesteatoma is a destructive and expanding growth of keratinizing squamous epithelium in the middle ear or petrous apex. The molecular and cellular processes of the pathogenesis of acquired middle ear cholesteatoma have not been fully understood. In this study, comparative proteomic analysis was conducted to investigate the roles of specific proteins in the pathways regarding keratinocyte proliferation in cholesteatoma. The differential proteins were detected by comparing the two-dimension electrophoresis (2-DE) maps of the epithelial tissues of 12 attic cholesteatomas with those of retroauricular skins. There were 14 upregulated proteins in the epithelial tissues of cholesteatoma in comparison with retroauricular skin. The modulation of five crucial proteins, HSP27, PRDX2, GRP75, GRP78 and GRP94, was further determined by RT-PCR, Western blot and immunohistochemistry. Phosphorylation of HSP27 at Ser-82 was identified by mass spectroscopy. The results of this study suggested that phosphorylated HSP27 is the end expression of two potential signal-transduction pathways, and together with PRDX2, they are very likely involved in the proliferation of keratinocytes in cholesteatoma. Upregulations of GRP75, GRP78 and GRP94 in keratinocytes may be able to counter endoplasmic reticulum stress, to inhibit cell apoptosis, to prevent protein unfolding and to promote cholesteatoma growth.
publisher	Molecular Diversity Preservation International (MDPI)
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3742253/
_version_	1612002753615233024

Upregulation of Phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in Acquired Middle Ear Cholesteatoma Growth

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