Hydrodynamic Delivery of Adiponectin and Adiponectin Receptor 2 Gene Blocks High-Fat Diet-Induced Obesity and Insulin Resistance

Hydrodynamic Delivery of Adiponectin and Adiponectin Receptor 2 Gene Blocks High-Fat Diet-Induced Obesity and Insulin Resistance

Adiponectin and its receptors are inversely related to the degree of obesity and have been identified as potential therapeutic targets for the treatment of obesity. In this study, we evaluated the effect of hydrodynamic delivery of adiponectin and/or its receptor 2 (adipoR2) genes on controlling the...

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Main Authors: Ma, Yongjie, Liu, Dexi
Format: Online
Language:English
Published: 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3740076/
id pubmed-3740076
recordtype oai_dc
spelling pubmed-37400762014-02-01 Hydrodynamic Delivery of Adiponectin and Adiponectin Receptor 2 Gene Blocks High-Fat Diet-Induced Obesity and Insulin Resistance Ma, Yongjie Liu, Dexi Article Adiponectin and its receptors are inversely related to the degree of obesity and have been identified as potential therapeutic targets for the treatment of obesity. In this study, we evaluated the effect of hydrodynamic delivery of adiponectin and/or its receptor 2 (adipoR2) genes on controlling the development of obesity and insulin resistance in AKR/J mice fed a high-fat diet. An increase in adiponectin and adipoR2 gene expression by hydrodynamic gene delivery prevented diet-induced weight gain, reduced fat accumulation in liver and adipose tissue, and improved insulin sensitivity. Beneficial effects were seen with reduced gluconeogenesis in the liver and lipogenesis in the liver, white adipose tissue and skeletal muscle. Real-time PCR analysis demonstrated overexpression of adiponectin and adipoR2 significantly suppressed transcription of phosphoenolpyruvate carboxykinase (pepck), glucose-6-phosphatase (g6pase), stearoyl CoA desaturase 1 (scd-1), and fatty acid synthase (fas) gene. Inhibition effects were mediated by activating the AMP-activated protein kinase (AMPK). These results prove that elevation of adiponectin and/or adipoR2 expression via gene transfer is an effective approach in managing obesity epidemics. 2013-02-21 2013-08 /pmc/articles/PMC3740076/ /pubmed/23425917 http://dx.doi.org/10.1038/gt.2013.8 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Ma, Yongjie
Liu, Dexi
spellingShingle Ma, Yongjie
Liu, Dexi
Hydrodynamic Delivery of Adiponectin and Adiponectin Receptor 2 Gene Blocks High-Fat Diet-Induced Obesity and Insulin Resistance
author_facet Ma, Yongjie
Liu, Dexi
author_sort Ma, Yongjie
title Hydrodynamic Delivery of Adiponectin and Adiponectin Receptor 2 Gene Blocks High-Fat Diet-Induced Obesity and Insulin Resistance
title_short Hydrodynamic Delivery of Adiponectin and Adiponectin Receptor 2 Gene Blocks High-Fat Diet-Induced Obesity and Insulin Resistance
title_full Hydrodynamic Delivery of Adiponectin and Adiponectin Receptor 2 Gene Blocks High-Fat Diet-Induced Obesity and Insulin Resistance
title_fullStr Hydrodynamic Delivery of Adiponectin and Adiponectin Receptor 2 Gene Blocks High-Fat Diet-Induced Obesity and Insulin Resistance
title_full_unstemmed Hydrodynamic Delivery of Adiponectin and Adiponectin Receptor 2 Gene Blocks High-Fat Diet-Induced Obesity and Insulin Resistance
title_sort hydrodynamic delivery of adiponectin and adiponectin receptor 2 gene blocks high-fat diet-induced obesity and insulin resistance
description Adiponectin and its receptors are inversely related to the degree of obesity and have been identified as potential therapeutic targets for the treatment of obesity. In this study, we evaluated the effect of hydrodynamic delivery of adiponectin and/or its receptor 2 (adipoR2) genes on controlling the development of obesity and insulin resistance in AKR/J mice fed a high-fat diet. An increase in adiponectin and adipoR2 gene expression by hydrodynamic gene delivery prevented diet-induced weight gain, reduced fat accumulation in liver and adipose tissue, and improved insulin sensitivity. Beneficial effects were seen with reduced gluconeogenesis in the liver and lipogenesis in the liver, white adipose tissue and skeletal muscle. Real-time PCR analysis demonstrated overexpression of adiponectin and adipoR2 significantly suppressed transcription of phosphoenolpyruvate carboxykinase (pepck), glucose-6-phosphatase (g6pase), stearoyl CoA desaturase 1 (scd-1), and fatty acid synthase (fas) gene. Inhibition effects were mediated by activating the AMP-activated protein kinase (AMPK). These results prove that elevation of adiponectin and/or adipoR2 expression via gene transfer is an effective approach in managing obesity epidemics.
publishDate 2013
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3740076/
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