Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA

Expression of chitinase is developmentally regulated in insects in consonance with their molting process. During the larval-larval metamorphosis in Helicoverpa armigera, chitinase gene expression varies from high to negligible. In the five-day metamorphic course of fifth-instar larvae, chitinase tra...

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Main Authors:	Agrawal, Neema, Sachdev, Bindiya, Rodrigues, Janneth, Sree, K. Sowjanya, Bhatnagar, Raj K.
Format:	Online
Language:	English
Published:	Nature Publishing Group 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3724176/

id	pubmed-3724176
recordtype	oai_dc
spelling	pubmed-37241762013-07-26 Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA Agrawal, Neema Sachdev, Bindiya Rodrigues, Janneth Sree, K. Sowjanya Bhatnagar, Raj K. Article Expression of chitinase is developmentally regulated in insects in consonance with their molting process. During the larval-larval metamorphosis in Helicoverpa armigera, chitinase gene expression varies from high to negligible. In the five-day metamorphic course of fifth-instar larvae, chitinase transcript is least abundant on third day and maximal on fifth day. MicroRNA library prepared from these highest and lowest chitinase-expressing larval stages resulted in isolation of several miRNAs. In silico analysis of sequenced miRNAs revealed three miRNAs having sequence similarity to 3′UTR of chitinase. Gene-targeted specific action of these miRNAs, was investigated by luciferase reporter having 3′UTR of chitinase. Only one of three miRNAs, miR-24, inhibited luciferase expression. Further, a day-wise in vivo quantification of miR-24 in fifth-instar larvae revealed a negative correlation with corresponding chitinase transcript abundance. The force-feeding of synthetic miR-24 induced significant morphological aberrations accompanied with arrest of molting. These miR-24 force-fed larvae revealed significantly reduced chitinase transcript abundance. Nature Publishing Group 2013-07-26 /pmc/articles/PMC3724176/ /pubmed/23887392 http://dx.doi.org/10.1038/srep02292 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Agrawal, Neema Sachdev, Bindiya Rodrigues, Janneth Sree, K. Sowjanya Bhatnagar, Raj K.
spellingShingle	Agrawal, Neema Sachdev, Bindiya Rodrigues, Janneth Sree, K. Sowjanya Bhatnagar, Raj K. Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA
author_facet	Agrawal, Neema Sachdev, Bindiya Rodrigues, Janneth Sree, K. Sowjanya Bhatnagar, Raj K.
author_sort	Agrawal, Neema
title	Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA
title_short	Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA
title_full	Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA
title_fullStr	Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA
title_full_unstemmed	Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA
title_sort	development associated profiling of chitinase and microrna of helicoverpa armigera identified chitinase repressive microrna
description	Expression of chitinase is developmentally regulated in insects in consonance with their molting process. During the larval-larval metamorphosis in Helicoverpa armigera, chitinase gene expression varies from high to negligible. In the five-day metamorphic course of fifth-instar larvae, chitinase transcript is least abundant on third day and maximal on fifth day. MicroRNA library prepared from these highest and lowest chitinase-expressing larval stages resulted in isolation of several miRNAs. In silico analysis of sequenced miRNAs revealed three miRNAs having sequence similarity to 3′UTR of chitinase. Gene-targeted specific action of these miRNAs, was investigated by luciferase reporter having 3′UTR of chitinase. Only one of three miRNAs, miR-24, inhibited luciferase expression. Further, a day-wise in vivo quantification of miR-24 in fifth-instar larvae revealed a negative correlation with corresponding chitinase transcript abundance. The force-feeding of synthetic miR-24 induced significant morphological aberrations accompanied with arrest of molting. These miR-24 force-fed larvae revealed significantly reduced chitinase transcript abundance.
publisher	Nature Publishing Group
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3724176/
_version_	1611998230893035520

Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA

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