Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus
Adult hippocampal neurogenesis results in the continuous formation of new neurons and is a process of brain plasticity involved in learning and memory. Although inducible-reversible transgenic mouse models are increasingly being used to investigate adult neurogenesis, transgene control requires the...
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| Format: | Online |
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Frontiers Media S.A.
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722480/ |
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pubmed-37224802013-07-29 Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus Sultan, Sebastien Gebara, Elias Toni, Nicolas Neuroscience Adult hippocampal neurogenesis results in the continuous formation of new neurons and is a process of brain plasticity involved in learning and memory. Although inducible-reversible transgenic mouse models are increasingly being used to investigate adult neurogenesis, transgene control requires the administration of an activator, doxycycline (Dox), with unknown effects on adult neurogenesis. Here, we tested the effect of Dox administration on adult neurogenesis in vivo. We found that 4 weeks of Dox treatment at doses commonly used for gene expression control, resulted in increased neurogenesis. Furthermore, the dendrites of new neurons displayed increased spine density. Concomitantly, Iba1-expressing microglia was reduced by Dox treatment. These results indicate that Dox treatment may interfere with parameters of relevance for the use of inducible transgenic mice in studies of adult neurogenesis or brain inflammation. Frontiers Media S.A. 2013-07-25 /pmc/articles/PMC3722480/ /pubmed/23898238 http://dx.doi.org/10.3389/fnins.2013.00131 Text en Copyright © 2013 Sultan, Gebara and Toni. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Sultan, Sebastien Gebara, Elias Toni, Nicolas |
| spellingShingle |
Sultan, Sebastien Gebara, Elias Toni, Nicolas Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus |
| author_facet |
Sultan, Sebastien Gebara, Elias Toni, Nicolas |
| author_sort |
Sultan, Sebastien |
| title |
Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus |
| title_short |
Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus |
| title_full |
Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus |
| title_fullStr |
Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus |
| title_full_unstemmed |
Doxycycline increases neurogenesis and reduces microglia in the adult hippocampus |
| title_sort |
doxycycline increases neurogenesis and reduces microglia in the adult hippocampus |
| description |
Adult hippocampal neurogenesis results in the continuous formation of new neurons and is a process of brain plasticity involved in learning and memory. Although inducible-reversible transgenic mouse models are increasingly being used to investigate adult neurogenesis, transgene control requires the administration of an activator, doxycycline (Dox), with unknown effects on adult neurogenesis. Here, we tested the effect of Dox administration on adult neurogenesis in vivo. We found that 4 weeks of Dox treatment at doses commonly used for gene expression control, resulted in increased neurogenesis. Furthermore, the dendrites of new neurons displayed increased spine density. Concomitantly, Iba1-expressing microglia was reduced by Dox treatment. These results indicate that Dox treatment may interfere with parameters of relevance for the use of inducible transgenic mice in studies of adult neurogenesis or brain inflammation. |
| publisher |
Frontiers Media S.A. |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3722480/ |
| _version_ |
1611997721712918528 |