Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP

Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP

There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here...

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Main Authors: Cheun, Hyeng-Il, Kim, Kyungjin, Yoon, Sejoung, Lee, Won-Ja, Park, Woo-Yoon, Sim, Seobo, Yu, Jae-Ran
Format: Online
Language:English
Published: The Korean Society for Parasitology and Tropical Medicine 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712111/
id pubmed-3712111
recordtype oai_dc
spelling pubmed-37121112013-07-17 Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP Cheun, Hyeng-Il Kim, Kyungjin Yoon, Sejoung Lee, Won-Ja Park, Woo-Yoon Sim, Seobo Yu, Jae-Ran Brief Communication There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections. The Korean Society for Parasitology and Tropical Medicine 2013-06 2013-06-30 /pmc/articles/PMC3712111/ /pubmed/23864748 http://dx.doi.org/10.3347/kjp.2013.51.3.353 Text en © 2013, Korean Society for Parasitology and Tropical Medicine http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Cheun, Hyeng-Il
Kim, Kyungjin
Yoon, Sejoung
Lee, Won-Ja
Park, Woo-Yoon
Sim, Seobo
Yu, Jae-Ran
spellingShingle Cheun, Hyeng-Il
Kim, Kyungjin
Yoon, Sejoung
Lee, Won-Ja
Park, Woo-Yoon
Sim, Seobo
Yu, Jae-Ran
Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
author_facet Cheun, Hyeng-Il
Kim, Kyungjin
Yoon, Sejoung
Lee, Won-Ja
Park, Woo-Yoon
Sim, Seobo
Yu, Jae-Ran
author_sort Cheun, Hyeng-Il
title Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
title_short Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
title_full Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
title_fullStr Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
title_full_unstemmed Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP
title_sort cryptosporidium hominis infection diagnosed by real-time pcr-rflp
description There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections.
publisher The Korean Society for Parasitology and Tropical Medicine
publishDate 2013
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3712111/
_version_ 1611995003799732224

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