Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from Brucella spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow’s Milk Proteins
The discovery of novel mucosal adjuvants will help to develop new formulations to control infectious and allergic diseases. In this work we demonstrate that U-Omp16 from Brucella spp. delivered by the nasal route (i.n.) induced an inflammatory immune response in bronchoalveolar lavage (BAL) and lu...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Public Library of Science
2013
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703917/ |
| id |
pubmed-3703917 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-37039172013-07-16 Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from Brucella spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow’s Milk Proteins Ibañez, Andrés E. Smaldini, Paola Coria, Lorena M. Delpino, María V. Pacífico, Lucila G. G. Oliveira, Sergio C. Risso, Gabriela S. Pasquevich, Karina A. Fossati, Carlos Alberto Giambartolomei, Guillermo H. Docena, Guillermo H. Cassataro, Juliana Research Article The discovery of novel mucosal adjuvants will help to develop new formulations to control infectious and allergic diseases. In this work we demonstrate that U-Omp16 from Brucella spp. delivered by the nasal route (i.n.) induced an inflammatory immune response in bronchoalveolar lavage (BAL) and lung tissues. Nasal co-administration of U-Omp16 with the model antigen (Ag) ovalbumin (OVA) increased the amount of Ag in lung tissues and induced OVA-specific systemic IgG and T helper (Th) 1 immune responses. The usefulness of U-Omp16 was also assessed in a mouse model of food allergy. U-Omp16 i.n. administration during sensitization ameliorated the hypersensitivity responses of sensitized mice upon oral exposure to Cow’s Milk Protein (CMP), decreased clinical signs, reduced anti-CMP IgE serum antibodies and modulated the Th2 response in favor of Th1 immunity. Thus, U-Omp16 could be used as a broad Th1 mucosal adjuvant for different Ag formulations. Public Library of Science 2013-07-05 /pmc/articles/PMC3703917/ /pubmed/23861971 http://dx.doi.org/10.1371/journal.pone.0069438 Text en © 2013 Ibañez et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Ibañez, Andrés E. Smaldini, Paola Coria, Lorena M. Delpino, María V. Pacífico, Lucila G. G. Oliveira, Sergio C. Risso, Gabriela S. Pasquevich, Karina A. Fossati, Carlos Alberto Giambartolomei, Guillermo H. Docena, Guillermo H. Cassataro, Juliana |
| spellingShingle |
Ibañez, Andrés E. Smaldini, Paola Coria, Lorena M. Delpino, María V. Pacífico, Lucila G. G. Oliveira, Sergio C. Risso, Gabriela S. Pasquevich, Karina A. Fossati, Carlos Alberto Giambartolomei, Guillermo H. Docena, Guillermo H. Cassataro, Juliana Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from Brucella spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow’s Milk Proteins |
| author_facet |
Ibañez, Andrés E. Smaldini, Paola Coria, Lorena M. Delpino, María V. Pacífico, Lucila G. G. Oliveira, Sergio C. Risso, Gabriela S. Pasquevich, Karina A. Fossati, Carlos Alberto Giambartolomei, Guillermo H. Docena, Guillermo H. Cassataro, Juliana |
| author_sort |
Ibañez, Andrés E. |
| title |
Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from
Brucella
spp. as Nasal Adjuvant Induces a
Th1 Immune Response and Modulates the Th2 Allergic Response to Cow’s Milk
Proteins |
| title_short |
Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from
Brucella
spp. as Nasal Adjuvant Induces a
Th1 Immune Response and Modulates the Th2 Allergic Response to Cow’s Milk
Proteins |
| title_full |
Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from
Brucella
spp. as Nasal Adjuvant Induces a
Th1 Immune Response and Modulates the Th2 Allergic Response to Cow’s Milk
Proteins |
| title_fullStr |
Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from
Brucella
spp. as Nasal Adjuvant Induces a
Th1 Immune Response and Modulates the Th2 Allergic Response to Cow’s Milk
Proteins |
| title_full_unstemmed |
Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from
Brucella
spp. as Nasal Adjuvant Induces a
Th1 Immune Response and Modulates the Th2 Allergic Response to Cow’s Milk
Proteins |
| title_sort |
unlipidated outer membrane protein omp16 (u-omp16) from
brucella
spp. as nasal adjuvant induces a
th1 immune response and modulates the th2 allergic response to cow’s milk
proteins |
| description |
The discovery of novel mucosal adjuvants will help to develop new formulations to
control infectious and allergic diseases. In this work we demonstrate that
U-Omp16 from
Brucella
spp. delivered by the nasal
route (i.n.) induced an inflammatory immune response in bronchoalveolar lavage
(BAL) and lung tissues. Nasal co-administration of U-Omp16 with the model
antigen (Ag) ovalbumin (OVA) increased the amount of Ag in lung tissues and
induced OVA-specific systemic IgG and T helper (Th) 1 immune responses. The
usefulness of U-Omp16 was also assessed in a mouse model of food allergy.
U-Omp16 i.n. administration during sensitization ameliorated the
hypersensitivity responses of sensitized mice upon oral exposure to Cow’s Milk
Protein (CMP), decreased clinical signs, reduced anti-CMP IgE serum antibodies
and modulated the Th2 response in favor of Th1 immunity. Thus, U-Omp16 could be
used as a broad Th1 mucosal adjuvant for different Ag formulations. |
| publisher |
Public Library of Science |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703917/ |
| _version_ |
1611992579140747264 |