The genome sequence of the colonial chordate, Botryllus schlosseri
Botryllus schlosseri is a colonial urochordate that follows the chordate plan of development following sexual reproduction, but invokes a stem cell-mediated budding program during subsequent rounds of asexual reproduction. As urochordates are considered to be the closest living invertebrate relative...
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| Format: | Online |
| Language: | English |
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eLife Sciences Publications, Ltd
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3699833/ |
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pubmed-3699833 |
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pubmed-36998332013-07-09 The genome sequence of the colonial chordate, Botryllus schlosseri Voskoboynik, Ayelet Neff, Norma F Sahoo, Debashis Newman, Aaron M Pushkarev, Dmitry Koh, Winston Passarelli, Benedetto Fan, H Christina Mantalas, Gary L Palmeri, Karla J Ishizuka, Katherine J Gissi, Carmela Griggio, Francesca Ben-Shlomo, Rachel Corey, Daniel M Penland, Lolita White, Richard A Weissman, Irving L Quake, Stephen R Developmental Biology and Stem Cells Botryllus schlosseri is a colonial urochordate that follows the chordate plan of development following sexual reproduction, but invokes a stem cell-mediated budding program during subsequent rounds of asexual reproduction. As urochordates are considered to be the closest living invertebrate relatives of vertebrates, they are ideal subjects for whole genome sequence analyses. Using a novel method for high-throughput sequencing of eukaryotic genomes, we sequenced and assembled 580 Mbp of the B. schlosseri genome. The genome assembly is comprised of nearly 14,000 intron-containing predicted genes, and 13,500 intron-less predicted genes, 40% of which could be confidently parceled into 13 (of 16 haploid) chromosomes. A comparison of homologous genes between B. schlosseri and other diverse taxonomic groups revealed genomic events underlying the evolution of vertebrates and lymphoid-mediated immunity. The B. schlosseri genome is a community resource for studying alternative modes of reproduction, natural transplantation reactions, and stem cell-mediated regeneration. eLife Sciences Publications, Ltd 2013-07-02 /pmc/articles/PMC3699833/ /pubmed/23840927 http://dx.doi.org/10.7554/eLife.00569 Text en © 2013, Voskoboynik et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Voskoboynik, Ayelet Neff, Norma F Sahoo, Debashis Newman, Aaron M Pushkarev, Dmitry Koh, Winston Passarelli, Benedetto Fan, H Christina Mantalas, Gary L Palmeri, Karla J Ishizuka, Katherine J Gissi, Carmela Griggio, Francesca Ben-Shlomo, Rachel Corey, Daniel M Penland, Lolita White, Richard A Weissman, Irving L Quake, Stephen R |
| spellingShingle |
Voskoboynik, Ayelet Neff, Norma F Sahoo, Debashis Newman, Aaron M Pushkarev, Dmitry Koh, Winston Passarelli, Benedetto Fan, H Christina Mantalas, Gary L Palmeri, Karla J Ishizuka, Katherine J Gissi, Carmela Griggio, Francesca Ben-Shlomo, Rachel Corey, Daniel M Penland, Lolita White, Richard A Weissman, Irving L Quake, Stephen R The genome sequence of the colonial chordate, Botryllus schlosseri |
| author_facet |
Voskoboynik, Ayelet Neff, Norma F Sahoo, Debashis Newman, Aaron M Pushkarev, Dmitry Koh, Winston Passarelli, Benedetto Fan, H Christina Mantalas, Gary L Palmeri, Karla J Ishizuka, Katherine J Gissi, Carmela Griggio, Francesca Ben-Shlomo, Rachel Corey, Daniel M Penland, Lolita White, Richard A Weissman, Irving L Quake, Stephen R |
| author_sort |
Voskoboynik, Ayelet |
| title |
The genome sequence of the colonial chordate, Botryllus schlosseri |
| title_short |
The genome sequence of the colonial chordate, Botryllus schlosseri |
| title_full |
The genome sequence of the colonial chordate, Botryllus schlosseri |
| title_fullStr |
The genome sequence of the colonial chordate, Botryllus schlosseri |
| title_full_unstemmed |
The genome sequence of the colonial chordate, Botryllus schlosseri |
| title_sort |
genome sequence of the colonial chordate, botryllus schlosseri |
| description |
Botryllus schlosseri is a colonial urochordate that follows the chordate plan of development following sexual reproduction, but invokes a stem cell-mediated budding program during subsequent rounds of asexual reproduction. As urochordates are considered to be the closest living invertebrate relatives of vertebrates, they are ideal subjects for whole genome sequence analyses. Using a novel method for high-throughput sequencing of eukaryotic genomes, we sequenced and assembled 580 Mbp of the B. schlosseri genome. The genome assembly is comprised of nearly 14,000 intron-containing predicted genes, and 13,500 intron-less predicted genes, 40% of which could be confidently parceled into 13 (of 16 haploid) chromosomes. A comparison of homologous genes between B. schlosseri and other diverse taxonomic groups revealed genomic events underlying the evolution of vertebrates and lymphoid-mediated immunity. The B. schlosseri genome is a community resource for studying alternative modes of reproduction, natural transplantation reactions, and stem cell-mediated regeneration. |
| publisher |
eLife Sciences Publications, Ltd |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3699833/ |
| _version_ |
1611991356114206720 |