DNA Methylation Regulated Nucleosome Dynamics

A strong correlation between nucleosome positioning and DNA methylation patterns has been reported in literature. However, the mechanistic model accounting for the correlation remains elusive. In this study, we evaluated the effects of specific DNA methylation patterns on modulating nucleosome confo...

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Main Authors:	Jimenez-Useche, Isabel, Ke, Jiaying, Tian, Yuqing, Shim, Daphne, Howell, Steven C., Qiu, Xiangyun, Yuan, Chongli
Format:	Online
Language:	English
Published:	Nature Publishing Group 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3698496/

id	pubmed-3698496
recordtype	oai_dc
spelling	pubmed-36984962013-07-03 DNA Methylation Regulated Nucleosome Dynamics Jimenez-Useche, Isabel Ke, Jiaying Tian, Yuqing Shim, Daphne Howell, Steven C. Qiu, Xiangyun Yuan, Chongli Article A strong correlation between nucleosome positioning and DNA methylation patterns has been reported in literature. However, the mechanistic model accounting for the correlation remains elusive. In this study, we evaluated the effects of specific DNA methylation patterns on modulating nucleosome conformation and stability using FRET and SAXS. CpG dinucleotide repeats at 10 bp intervals were found to play different roles in nucleosome stability dependent on their methylation states and their relative nucleosomal locations. An additional (CpG)5 stretch located in the nucleosomal central dyad does not alter the nucleosome conformation, but significant conformational differences were observed between the unmethylated and methylated nucleosomes. These findings suggest that the correlation between nucleosome positioning and DNA methylation patterns can arise from the variations in nucleosome stability dependent on their sequence and epigenetic content. This knowledge will help to reveal the detailed role of DNA methylation in regulating chromatin packaging and gene transcription. Nature Publishing Group 2013-07-02 /pmc/articles/PMC3698496/ /pubmed/23817195 http://dx.doi.org/10.1038/srep02121 Text en Copyright © 2013, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/3.0/ This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Jimenez-Useche, Isabel Ke, Jiaying Tian, Yuqing Shim, Daphne Howell, Steven C. Qiu, Xiangyun Yuan, Chongli
spellingShingle	Jimenez-Useche, Isabel Ke, Jiaying Tian, Yuqing Shim, Daphne Howell, Steven C. Qiu, Xiangyun Yuan, Chongli DNA Methylation Regulated Nucleosome Dynamics
author_facet	Jimenez-Useche, Isabel Ke, Jiaying Tian, Yuqing Shim, Daphne Howell, Steven C. Qiu, Xiangyun Yuan, Chongli
author_sort	Jimenez-Useche, Isabel
title	DNA Methylation Regulated Nucleosome Dynamics
title_short	DNA Methylation Regulated Nucleosome Dynamics
title_full	DNA Methylation Regulated Nucleosome Dynamics
title_fullStr	DNA Methylation Regulated Nucleosome Dynamics
title_full_unstemmed	DNA Methylation Regulated Nucleosome Dynamics
title_sort	dna methylation regulated nucleosome dynamics
description	A strong correlation between nucleosome positioning and DNA methylation patterns has been reported in literature. However, the mechanistic model accounting for the correlation remains elusive. In this study, we evaluated the effects of specific DNA methylation patterns on modulating nucleosome conformation and stability using FRET and SAXS. CpG dinucleotide repeats at 10 bp intervals were found to play different roles in nucleosome stability dependent on their methylation states and their relative nucleosomal locations. An additional (CpG)5 stretch located in the nucleosomal central dyad does not alter the nucleosome conformation, but significant conformational differences were observed between the unmethylated and methylated nucleosomes. These findings suggest that the correlation between nucleosome positioning and DNA methylation patterns can arise from the variations in nucleosome stability dependent on their sequence and epigenetic content. This knowledge will help to reveal the detailed role of DNA methylation in regulating chromatin packaging and gene transcription.
publisher	Nature Publishing Group
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3698496/
_version_	1611990989226901504

DNA Methylation Regulated Nucleosome Dynamics

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