Development and Evaluation of a Next-Generation Digital PCR Diagnostic Assay for Ocular Chlamydia trachomatis Infections

Development and Evaluation of a Next-Generation Digital PCR Diagnostic Assay for Ocular Chlamydia trachomatis Infections

Droplet digital PCR (ddPCR) is an emulsion PCR process that performs absolute quantitation of nucleic acids. We developed a ddPCR assay for Chlamydia trachomatis infections and found it to be accurate and precise. Using PCR mixtures containing plasmids engineered to include the PCR target sequences,...

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Bibliographic Details
Main Authors: Roberts, Chrissy h., Last, Anna, Molina-Gonzalez, Sandra, Cassama, Eunice, Butcher, Robert, Nabicassa, Meno, McCarthy, Elizabeth, Burr, Sarah E., Mabey, David C., Bailey, Robin L., Holland, Martin J.
Format: Online
Language:English
Published: American Society for Microbiology 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3697714/

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3697714/

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