Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation

Sm-like (Lsm) proteins have been identified in all organisms and are related to RNA metabolism. Here, we report that Arabidopsis nuclear AtLSM8 protein, as well as AtLSM5, which localizes to both the cytoplasm and nucleus, function in pre-mRNA splicing, while AtLSM5 and the exclusively cytoplasmic A...

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Main Authors:	Golisz, Anna, Sikorski, Pawel J., Kruszka, Katarzyna, Kufel, Joanna
Format:	Online
Language:	English
Published:	Oxford University Press 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695525/

id	pubmed-3695525
recordtype	oai_dc
spelling	pubmed-36955252013-06-28 Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation Golisz, Anna Sikorski, Pawel J. Kruszka, Katarzyna Kufel, Joanna RNA Sm-like (Lsm) proteins have been identified in all organisms and are related to RNA metabolism. Here, we report that Arabidopsis nuclear AtLSM8 protein, as well as AtLSM5, which localizes to both the cytoplasm and nucleus, function in pre-mRNA splicing, while AtLSM5 and the exclusively cytoplasmic AtLSM1 contribute to 5′–3′ mRNA decay. In lsm8 and sad1/lsm5 mutants, U6 small nuclear RNA (snRNA) was reduced and unspliced mRNA precursors accumulated, whereas mRNA stability was mainly affected in plants lacking AtLSM1 and AtLSM5. Some of the mRNAs affected in lsm1a lsm1b and sad1/lsm5 plants were also substrates of the cytoplasmic 5′–3′ exonuclease AtXRN4 and of the decapping enzyme AtDCP2. Surprisingly, a subset of substrates was also stabilized in the mutant lacking AtLSM8, which supports the notion that plant mRNAs are actively degraded in the nucleus. Localization of LSM components, purification of LSM-interacting proteins as well as functional analyses strongly suggest that at least two LSM complexes with conserved activities in RNA metabolism, AtLSM1-7 and AtLSM2-8, exist also in plants. Oxford University Press 2013-07 2013-04-24 /pmc/articles/PMC3695525/ /pubmed/23620288 http://dx.doi.org/10.1093/nar/gkt296 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Golisz, Anna Sikorski, Pawel J. Kruszka, Katarzyna Kufel, Joanna
spellingShingle	Golisz, Anna Sikorski, Pawel J. Kruszka, Katarzyna Kufel, Joanna Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation
author_facet	Golisz, Anna Sikorski, Pawel J. Kruszka, Katarzyna Kufel, Joanna
author_sort	Golisz, Anna
title	Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation
title_short	Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation
title_full	Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation
title_fullStr	Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation
title_full_unstemmed	Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation
title_sort	arabidopsis thaliana lsm proteins function in mrna splicing and degradation
description	Sm-like (Lsm) proteins have been identified in all organisms and are related to RNA metabolism. Here, we report that Arabidopsis nuclear AtLSM8 protein, as well as AtLSM5, which localizes to both the cytoplasm and nucleus, function in pre-mRNA splicing, while AtLSM5 and the exclusively cytoplasmic AtLSM1 contribute to 5′–3′ mRNA decay. In lsm8 and sad1/lsm5 mutants, U6 small nuclear RNA (snRNA) was reduced and unspliced mRNA precursors accumulated, whereas mRNA stability was mainly affected in plants lacking AtLSM1 and AtLSM5. Some of the mRNAs affected in lsm1a lsm1b and sad1/lsm5 plants were also substrates of the cytoplasmic 5′–3′ exonuclease AtXRN4 and of the decapping enzyme AtDCP2. Surprisingly, a subset of substrates was also stabilized in the mutant lacking AtLSM8, which supports the notion that plant mRNAs are actively degraded in the nucleus. Localization of LSM components, purification of LSM-interacting proteins as well as functional analyses strongly suggest that at least two LSM complexes with conserved activities in RNA metabolism, AtLSM1-7 and AtLSM2-8, exist also in plants.
publisher	Oxford University Press
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695525/
_version_	1611990297004212224

Arabidopsis thaliana LSM proteins function in mRNA splicing and degradation

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