Transfer and Expression of Small Interfering RNAs in Mammalian Cells Using Lentiviral Vectors

RNA interference is a convenient tool for modulating gene expression. The widespread application of RNA interference is made difficult because of the imperfections of the methods used for efficient target cell delivery of whatever genes are under study. One of the most convenient and efficient gene...

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Main Authors:	Lebedev, T. D., Spirin, P. V., Prassolov, V. S.
Format:	Online
Language:	English
Published:	A.I. Gordeyev 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695350/

id	pubmed-3695350
recordtype	oai_dc
spelling	pubmed-36953502013-07-01 Transfer and Expression of Small Interfering RNAs in Mammalian Cells Using Lentiviral Vectors Lebedev, T. D. Spirin, P. V. Prassolov, V. S. Review Article RNA interference is a convenient tool for modulating gene expression. The widespread application of RNA interference is made difficult because of the imperfections of the methods used for efficient target cell delivery of whatever genes are under study. One of the most convenient and efficient gene transfer and expression systems is based on the use of lentiviral vectors, which direct the synthesis of small hairpin RNAs (shRNAs), the precursors of siRNAs. The application of these systems enables one to achieve sustainable and long-term shRNA expression in cells. This review considers the adaptation of the processing of artificial shRNA to the mechanisms used by cellular microRNAs and simultaneous expression of several shRNAs as potential approaches for producing lentiviral vectors that direct shRNA synthesis. Approaches to using RNA interference for the treatment of cancer, as well as hereditary and viral diseases, are under active development today. The improvement made to the methods for constructing lentiviral vectors and the investigation into the mechanisms of processing of small interfering RNA allow one to now consider lentiviral vectors that direct shRNA synthesis as one of the most promising tools for delivering small interfering RNAs. A.I. Gordeyev 2013 /pmc/articles/PMC3695350/ /pubmed/23819033 Text en Copyright © 2013 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Lebedev, T. D. Spirin, P. V. Prassolov, V. S.
spellingShingle	Lebedev, T. D. Spirin, P. V. Prassolov, V. S. Transfer and Expression of Small Interfering RNAs in Mammalian Cells Using Lentiviral Vectors
author_facet	Lebedev, T. D. Spirin, P. V. Prassolov, V. S.
author_sort	Lebedev, T. D.
title	Transfer and Expression of Small Interfering RNAs in Mammalian Cells Using Lentiviral Vectors
title_short	Transfer and Expression of Small Interfering RNAs in Mammalian Cells Using Lentiviral Vectors
title_full	Transfer and Expression of Small Interfering RNAs in Mammalian Cells Using Lentiviral Vectors
title_fullStr	Transfer and Expression of Small Interfering RNAs in Mammalian Cells Using Lentiviral Vectors
title_full_unstemmed	Transfer and Expression of Small Interfering RNAs in Mammalian Cells Using Lentiviral Vectors
title_sort	transfer and expression of small interfering rnas in mammalian cells using lentiviral vectors
description	RNA interference is a convenient tool for modulating gene expression. The widespread application of RNA interference is made difficult because of the imperfections of the methods used for efficient target cell delivery of whatever genes are under study. One of the most convenient and efficient gene transfer and expression systems is based on the use of lentiviral vectors, which direct the synthesis of small hairpin RNAs (shRNAs), the precursors of siRNAs. The application of these systems enables one to achieve sustainable and long-term shRNA expression in cells. This review considers the adaptation of the processing of artificial shRNA to the mechanisms used by cellular microRNAs and simultaneous expression of several shRNAs as potential approaches for producing lentiviral vectors that direct shRNA synthesis. Approaches to using RNA interference for the treatment of cancer, as well as hereditary and viral diseases, are under active development today. The improvement made to the methods for constructing lentiviral vectors and the investigation into the mechanisms of processing of small interfering RNA allow one to now consider lentiviral vectors that direct shRNA synthesis as one of the most promising tools for delivering small interfering RNAs.
publisher	A.I. Gordeyev
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3695350/
_version_	1611990246230065152

Transfer and Expression of Small Interfering RNAs in Mammalian Cells Using Lentiviral Vectors

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