Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid
Noroviruses are major pathogens associated with acute gastroenteritis. They are diverse viruses, with at least six genogroups (GI-GVI) and multiple genotypes defined by differences in the major capsid protein, VP1. This diversity has challenged the development of broadly cross-reactive vaccines as w...
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| Format: | Online |
| Language: | English |
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Public Library of Science
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689733/ |
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pubmed-3689733 |
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pubmed-36897332013-06-26 Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid Parra, Gabriel I. Azure, JoLynn Fischer, Renate Bok, Karin Sandoval-Jaime, Carlos Sosnovtsev, Stanislav V. Sander, Peter Green, Kim Y. Research Article Noroviruses are major pathogens associated with acute gastroenteritis. They are diverse viruses, with at least six genogroups (GI-GVI) and multiple genotypes defined by differences in the major capsid protein, VP1. This diversity has challenged the development of broadly cross-reactive vaccines as well as efficient detection methods. Here, we report the characterization of a broadly cross-reactive monoclonal antibody (MAb) raised against the capsid protein of a GII.3 norovirus strain. The MAb reacted with VLPs and denatured VP1 protein from GI, GII, GIV and GV noroviruses, and mapped to a linear epitope located in the inner shell domain. An alignment of all available VP1 sequences showed that the putative epitope (residues 52–56) is highly conserved across the genus Norovirus. This broadly cross-reactive MAb thus constitutes a valuable reagent for the diagnosis and study of these diverse viruses. Public Library of Science 2013-06-21 /pmc/articles/PMC3689733/ /pubmed/23805319 http://dx.doi.org/10.1371/journal.pone.0067592 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Parra, Gabriel I. Azure, JoLynn Fischer, Renate Bok, Karin Sandoval-Jaime, Carlos Sosnovtsev, Stanislav V. Sander, Peter Green, Kim Y. |
| spellingShingle |
Parra, Gabriel I. Azure, JoLynn Fischer, Renate Bok, Karin Sandoval-Jaime, Carlos Sosnovtsev, Stanislav V. Sander, Peter Green, Kim Y. Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid |
| author_facet |
Parra, Gabriel I. Azure, JoLynn Fischer, Renate Bok, Karin Sandoval-Jaime, Carlos Sosnovtsev, Stanislav V. Sander, Peter Green, Kim Y. |
| author_sort |
Parra, Gabriel I. |
| title |
Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid |
| title_short |
Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid |
| title_full |
Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid |
| title_fullStr |
Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid |
| title_full_unstemmed |
Identification of a Broadly Cross-Reactive Epitope in the Inner Shell of the Norovirus Capsid |
| title_sort |
identification of a broadly cross-reactive epitope in the inner shell of the norovirus capsid |
| description |
Noroviruses are major pathogens associated with acute gastroenteritis. They are diverse viruses, with at least six genogroups (GI-GVI) and multiple genotypes defined by differences in the major capsid protein, VP1. This diversity has challenged the development of broadly cross-reactive vaccines as well as efficient detection methods. Here, we report the characterization of a broadly cross-reactive monoclonal antibody (MAb) raised against the capsid protein of a GII.3 norovirus strain. The MAb reacted with VLPs and denatured VP1 protein from GI, GII, GIV and GV noroviruses, and mapped to a linear epitope located in the inner shell domain. An alignment of all available VP1 sequences showed that the putative epitope (residues 52–56) is highly conserved across the genus Norovirus. This broadly cross-reactive MAb thus constitutes a valuable reagent for the diagnosis and study of these diverse viruses. |
| publisher |
Public Library of Science |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689733/ |
| _version_ |
1611988671479676928 |