Low Divergence of Clonorchis sinensis in China Based on Multilocus Analysis

Low Divergence of Clonorchis sinensis in China Based on Multilocus Analysis

Clonorchis sinensis, an ancient parasite that infects a number of piscivorous mammals, attracts significant public health interest due to zoonotic exposure risks in Asia. The available studies are insufficient to reflect the prevalence, geographic distribution, and intraspecific genetic diversity o...

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Main Authors: Sun, Jiufeng, Huang, Yan, Huang, Huaiqiu, Liang, Pei, Wang, Xiaoyun, Mao, Qiang, Men, Jingtao, Chen, Wenjun, Deng, Chuanhuan, Zhou, Chenhui, Lv, Xiaoli, Zhou, Juanjuan, Zhang, Fan, Li, Ran, Tian, Yanli, Lei, Huali, Liang, Chi, Hu, Xuchu, Xu, Jin, Li, Xuerong, XinbingYu
Format: Online
Language:English
Published: Public Library of Science 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688995/
id pubmed-3688995
recordtype oai_dc
spelling pubmed-36889952013-07-02 Low Divergence of Clonorchis sinensis in China Based on Multilocus Analysis Sun, Jiufeng Huang, Yan Huang, Huaiqiu Liang, Pei Wang, Xiaoyun Mao, Qiang Men, Jingtao Chen, Wenjun Deng, Chuanhuan Zhou, Chenhui Lv, Xiaoli Zhou, Juanjuan Zhang, Fan Li, Ran Tian, Yanli Lei, Huali Liang, Chi Hu, Xuchu Xu, Jin Li, Xuerong XinbingYu, Research Article Clonorchis sinensis, an ancient parasite that infects a number of piscivorous mammals, attracts significant public health interest due to zoonotic exposure risks in Asia. The available studies are insufficient to reflect the prevalence, geographic distribution, and intraspecific genetic diversity of C. sinensis in endemic areas. Here, a multilocus analysis based on eight genes (ITS1, act, tub, ef-1a, cox1, cox3, nad4 and nad5 [4.986 kb]) was employed to explore the intra-species genetic construction of C. sinensis in China. Two hundred and fifty-six C. sinensis isolates were obtained from environmental reservoirs from 17 provinces of China. A total of 254 recognized Multilocus Types (MSTs) showed high diversity among these isolates using multilocus analysis. The comparison analysis of nuclear and mitochondrial phylogeny supports separate clusters in a nuclear dendrogram. Genetic differentiation analysis of three clusters (A, B, and C) showed low divergence within populations. Most isolates from clusters B and C are geographically limited to central China, while cluster A is extraordinarily genetically diverse. Further genetic analyses between different geographic distributions, water bodies and hosts support the low population divergence. The latter haplotype analyses were consistent with the phylogenetic and genetic differentiation results. A recombination network based on concatenated sequences showed a concentrated linkage recombination population in cox1, cox3, nad4 and nad5, with spatial structuring in ITS1. Coupled with the history record and archaeological evidence of C. sinensis infection in mummified desiccated feces, these data point to an ancient origin of C. sinensis in China. In conclusion, we present a likely phylogenetic structure of the C. sinensis population in mainland China, highlighting its possible tendency for biogeographic expansion. Meanwhile, ITS1 was found to be an effective marker for tracking C. sinensis infection worldwide. Thus, the present study improves our understanding of the global epidemiology and evolution of C. sinensis. Public Library of Science 2013-06-18 /pmc/articles/PMC3688995/ /pubmed/23825605 http://dx.doi.org/10.1371/journal.pone.0067006 Text en © 2013 Sun et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Sun, Jiufeng
Huang, Yan
Huang, Huaiqiu
Liang, Pei
Wang, Xiaoyun
Mao, Qiang
Men, Jingtao
Chen, Wenjun
Deng, Chuanhuan
Zhou, Chenhui
Lv, Xiaoli
Zhou, Juanjuan
Zhang, Fan
Li, Ran
Tian, Yanli
Lei, Huali
Liang, Chi
Hu, Xuchu
Xu, Jin
Li, Xuerong
XinbingYu,
spellingShingle Sun, Jiufeng
Huang, Yan
Huang, Huaiqiu
Liang, Pei
Wang, Xiaoyun
Mao, Qiang
Men, Jingtao
Chen, Wenjun
Deng, Chuanhuan
Zhou, Chenhui
Lv, Xiaoli
Zhou, Juanjuan
Zhang, Fan
Li, Ran
Tian, Yanli
Lei, Huali
Liang, Chi
Hu, Xuchu
Xu, Jin
Li, Xuerong
XinbingYu,
Low Divergence of Clonorchis sinensis in China Based on Multilocus Analysis
author_facet Sun, Jiufeng
Huang, Yan
Huang, Huaiqiu
Liang, Pei
Wang, Xiaoyun
Mao, Qiang
Men, Jingtao
Chen, Wenjun
Deng, Chuanhuan
Zhou, Chenhui
Lv, Xiaoli
Zhou, Juanjuan
Zhang, Fan
Li, Ran
Tian, Yanli
Lei, Huali
Liang, Chi
Hu, Xuchu
Xu, Jin
Li, Xuerong
XinbingYu,
author_sort Sun, Jiufeng
title Low Divergence of Clonorchis sinensis in China Based on Multilocus Analysis
title_short Low Divergence of Clonorchis sinensis in China Based on Multilocus Analysis
title_full Low Divergence of Clonorchis sinensis in China Based on Multilocus Analysis
title_fullStr Low Divergence of Clonorchis sinensis in China Based on Multilocus Analysis
title_full_unstemmed Low Divergence of Clonorchis sinensis in China Based on Multilocus Analysis
title_sort low divergence of clonorchis sinensis in china based on multilocus analysis
description Clonorchis sinensis, an ancient parasite that infects a number of piscivorous mammals, attracts significant public health interest due to zoonotic exposure risks in Asia. The available studies are insufficient to reflect the prevalence, geographic distribution, and intraspecific genetic diversity of C. sinensis in endemic areas. Here, a multilocus analysis based on eight genes (ITS1, act, tub, ef-1a, cox1, cox3, nad4 and nad5 [4.986 kb]) was employed to explore the intra-species genetic construction of C. sinensis in China. Two hundred and fifty-six C. sinensis isolates were obtained from environmental reservoirs from 17 provinces of China. A total of 254 recognized Multilocus Types (MSTs) showed high diversity among these isolates using multilocus analysis. The comparison analysis of nuclear and mitochondrial phylogeny supports separate clusters in a nuclear dendrogram. Genetic differentiation analysis of three clusters (A, B, and C) showed low divergence within populations. Most isolates from clusters B and C are geographically limited to central China, while cluster A is extraordinarily genetically diverse. Further genetic analyses between different geographic distributions, water bodies and hosts support the low population divergence. The latter haplotype analyses were consistent with the phylogenetic and genetic differentiation results. A recombination network based on concatenated sequences showed a concentrated linkage recombination population in cox1, cox3, nad4 and nad5, with spatial structuring in ITS1. Coupled with the history record and archaeological evidence of C. sinensis infection in mummified desiccated feces, these data point to an ancient origin of C. sinensis in China. In conclusion, we present a likely phylogenetic structure of the C. sinensis population in mainland China, highlighting its possible tendency for biogeographic expansion. Meanwhile, ITS1 was found to be an effective marker for tracking C. sinensis infection worldwide. Thus, the present study improves our understanding of the global epidemiology and evolution of C. sinensis.
publisher Public Library of Science
publishDate 2013
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688995/
_version_ 1611988472822759424

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