Intraflagellar transport drives flagellar surface motility

The assembly and maintenance of all cilia and flagella require intraflagellar transport (IFT) along the axoneme. IFT has been implicated in sensory and motile ciliary functions, but the mechanisms of this relationship remain unclear. Here, we used Chlamydomonas flagellar surface motility (FSM) as a...

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Main Authors:	Shih, Sheng Min, Engel, Benjamin D, Kocabas, Fatih, Bilyard, Thomas, Gennerich, Arne, Marshall, Wallace F, Yildiz, Ahmet
Format:	Online
Language:	English
Published:	eLife Sciences Publications, Ltd 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679542/

id	pubmed-3679542
recordtype	oai_dc
spelling	pubmed-36795422013-06-21 Intraflagellar transport drives flagellar surface motility Shih, Sheng Min Engel, Benjamin D Kocabas, Fatih Bilyard, Thomas Gennerich, Arne Marshall, Wallace F Yildiz, Ahmet Biophysics and Structural Biology The assembly and maintenance of all cilia and flagella require intraflagellar transport (IFT) along the axoneme. IFT has been implicated in sensory and motile ciliary functions, but the mechanisms of this relationship remain unclear. Here, we used Chlamydomonas flagellar surface motility (FSM) as a model to test whether IFT provides force for gliding of cells across solid surfaces. We show that IFT trains are coupled to flagellar membrane glycoproteins (FMGs) in a Ca2+-dependent manner. IFT trains transiently pause through surface adhesion of their FMG cargos, and dynein-1b motors pull the cell towards the distal tip of the axoneme. Each train is transported by at least four motors, with only one type of motor active at a time. Our results demonstrate the mechanism of Chlamydomonas gliding motility and suggest that IFT plays a major role in adhesion-induced ciliary signaling pathways. eLife Sciences Publications, Ltd 2013-06-11 /pmc/articles/PMC3679542/ /pubmed/23795295 http://dx.doi.org/10.7554/eLife.00744 Text en Copyright © 2013, Shih et al http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Shih, Sheng Min Engel, Benjamin D Kocabas, Fatih Bilyard, Thomas Gennerich, Arne Marshall, Wallace F Yildiz, Ahmet
spellingShingle	Shih, Sheng Min Engel, Benjamin D Kocabas, Fatih Bilyard, Thomas Gennerich, Arne Marshall, Wallace F Yildiz, Ahmet Intraflagellar transport drives flagellar surface motility
author_facet	Shih, Sheng Min Engel, Benjamin D Kocabas, Fatih Bilyard, Thomas Gennerich, Arne Marshall, Wallace F Yildiz, Ahmet
author_sort	Shih, Sheng Min
title	Intraflagellar transport drives flagellar surface motility
title_short	Intraflagellar transport drives flagellar surface motility
title_full	Intraflagellar transport drives flagellar surface motility
title_fullStr	Intraflagellar transport drives flagellar surface motility
title_full_unstemmed	Intraflagellar transport drives flagellar surface motility
title_sort	intraflagellar transport drives flagellar surface motility
description	The assembly and maintenance of all cilia and flagella require intraflagellar transport (IFT) along the axoneme. IFT has been implicated in sensory and motile ciliary functions, but the mechanisms of this relationship remain unclear. Here, we used Chlamydomonas flagellar surface motility (FSM) as a model to test whether IFT provides force for gliding of cells across solid surfaces. We show that IFT trains are coupled to flagellar membrane glycoproteins (FMGs) in a Ca2+-dependent manner. IFT trains transiently pause through surface adhesion of their FMG cargos, and dynein-1b motors pull the cell towards the distal tip of the axoneme. Each train is transported by at least four motors, with only one type of motor active at a time. Our results demonstrate the mechanism of Chlamydomonas gliding motility and suggest that IFT plays a major role in adhesion-induced ciliary signaling pathways.
publisher	eLife Sciences Publications, Ltd
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679542/
_version_	1611985561404309504

Intraflagellar transport drives flagellar surface motility

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