Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling

Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling

Caffeic acid phenethyl ester (CAPE) is a bioactive component extracted from honeybee hive propolis. Our observations indicated that CAPE treatment suppressed cell proliferation and colony formation of TW2.6 human oral squamous cell carcinoma (OSCC) cells dose-dependently. CAPE treatment decreased G1...

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Main Authors: Kuo, Ying-Yu, Lin, Hui-Ping, Huo, Chieh, Su, Liang-Cheng, Yang, Jonathan, Hsiao, Ping-Hsuan, Chiang, Hung-Che, Chung, Chi-Jung, Wang, Horng-Dar, Chang, Jang-Yang, Chen, Ya-Wen, Chuu, Chih-Pin
Format: Online
Language:English
Published: Molecular Diversity Preservation International (MDPI) 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676757/
id pubmed-3676757
recordtype oai_dc
spelling pubmed-36767572013-07-02 Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling Kuo, Ying-Yu Lin, Hui-Ping Huo, Chieh Su, Liang-Cheng Yang, Jonathan Hsiao, Ping-Hsuan Chiang, Hung-Che Chung, Chi-Jung Wang, Horng-Dar Chang, Jang-Yang Chen, Ya-Wen Chuu, Chih-Pin Article Caffeic acid phenethyl ester (CAPE) is a bioactive component extracted from honeybee hive propolis. Our observations indicated that CAPE treatment suppressed cell proliferation and colony formation of TW2.6 human oral squamous cell carcinoma (OSCC) cells dose-dependently. CAPE treatment decreased G1 phase cell population, increased G2/M phase cell population, and induced apoptosis in TW2.6 cells. Treatment with CAPE decreased protein abundance of Akt, Akt1, Akt2, Akt3, phospho-Akt Ser473, phospho-Akt Thr 308, GSK3β, FOXO1, FOXO3a, phospho-FOXO1 Thr24, phospho-FoxO3a Thr32, NF-κB, phospho-NF-κB Ser536, Rb, phospho-Rb Ser807/811, Skp2, and cyclin D1, but increased cell cycle inhibitor p27Kip. Overexpression of Akt1 or Akt2 in TW2.6 cells rescued growth inhibition caused by CAPE treatment. Co-treating TW2.6 cells with CAPE and 5-fluorouracil, a commonly used chemotherapeutic drug for oral cancers, exhibited additive cell proliferation inhibition. Our study suggested that administration of CAPE is a potential adjuvant therapy for patients with OSCC oral cancer. Molecular Diversity Preservation International (MDPI) 2013-04-24 /pmc/articles/PMC3676757/ /pubmed/23615471 http://dx.doi.org/10.3390/ijms14058801 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type Open Access Journal
institution_category Foreign Institution
institution US National Center for Biotechnology Information
building NCBI PubMed
collection Online Access
language English
format Online
author Kuo, Ying-Yu
Lin, Hui-Ping
Huo, Chieh
Su, Liang-Cheng
Yang, Jonathan
Hsiao, Ping-Hsuan
Chiang, Hung-Che
Chung, Chi-Jung
Wang, Horng-Dar
Chang, Jang-Yang
Chen, Ya-Wen
Chuu, Chih-Pin
spellingShingle Kuo, Ying-Yu
Lin, Hui-Ping
Huo, Chieh
Su, Liang-Cheng
Yang, Jonathan
Hsiao, Ping-Hsuan
Chiang, Hung-Che
Chung, Chi-Jung
Wang, Horng-Dar
Chang, Jang-Yang
Chen, Ya-Wen
Chuu, Chih-Pin
Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling
author_facet Kuo, Ying-Yu
Lin, Hui-Ping
Huo, Chieh
Su, Liang-Cheng
Yang, Jonathan
Hsiao, Ping-Hsuan
Chiang, Hung-Che
Chung, Chi-Jung
Wang, Horng-Dar
Chang, Jang-Yang
Chen, Ya-Wen
Chuu, Chih-Pin
author_sort Kuo, Ying-Yu
title Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling
title_short Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling
title_full Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling
title_fullStr Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling
title_full_unstemmed Caffeic Acid Phenethyl Ester Suppresses Proliferation and Survival of TW2.6 Human Oral Cancer Cells via Inhibition of Akt Signaling
title_sort caffeic acid phenethyl ester suppresses proliferation and survival of tw2.6 human oral cancer cells via inhibition of akt signaling
description Caffeic acid phenethyl ester (CAPE) is a bioactive component extracted from honeybee hive propolis. Our observations indicated that CAPE treatment suppressed cell proliferation and colony formation of TW2.6 human oral squamous cell carcinoma (OSCC) cells dose-dependently. CAPE treatment decreased G1 phase cell population, increased G2/M phase cell population, and induced apoptosis in TW2.6 cells. Treatment with CAPE decreased protein abundance of Akt, Akt1, Akt2, Akt3, phospho-Akt Ser473, phospho-Akt Thr 308, GSK3β, FOXO1, FOXO3a, phospho-FOXO1 Thr24, phospho-FoxO3a Thr32, NF-κB, phospho-NF-κB Ser536, Rb, phospho-Rb Ser807/811, Skp2, and cyclin D1, but increased cell cycle inhibitor p27Kip. Overexpression of Akt1 or Akt2 in TW2.6 cells rescued growth inhibition caused by CAPE treatment. Co-treating TW2.6 cells with CAPE and 5-fluorouracil, a commonly used chemotherapeutic drug for oral cancers, exhibited additive cell proliferation inhibition. Our study suggested that administration of CAPE is a potential adjuvant therapy for patients with OSCC oral cancer.
publisher Molecular Diversity Preservation International (MDPI)
publishDate 2013
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676757/
_version_ 1611984783453192192

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