Hsc70 Is a Novel Interactor of NF-kappaB p65 in Living Hippocampal Neurons

Signaling via NF-κB in neurons depends on complex formation with interactors such as dynein/dynactin motor complex and can be triggered by synaptic activation. However, so far a detailed interaction map for the neuronal NF-κB is missing. In this study we used mass spectrometry to identify novel inte...

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Main Authors:	Klenke, Christin, Widera, Darius, Engelen, Thomas, Müller, Janine, Noll, Thomas, Niehaus, Karsten, Schmitz, M. Lienhard, Kaltschmidt, Barbara, Kaltschmidt, Christian
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676459/

id	pubmed-3676459
recordtype	oai_dc
spelling	pubmed-36764592013-06-12 Hsc70 Is a Novel Interactor of NF-kappaB p65 in Living Hippocampal Neurons Klenke, Christin Widera, Darius Engelen, Thomas Müller, Janine Noll, Thomas Niehaus, Karsten Schmitz, M. Lienhard Kaltschmidt, Barbara Kaltschmidt, Christian Research Article Signaling via NF-κB in neurons depends on complex formation with interactors such as dynein/dynactin motor complex and can be triggered by synaptic activation. However, so far a detailed interaction map for the neuronal NF-κB is missing. In this study we used mass spectrometry to identify novel interactors of NF-κB p65 within the brain. Hsc70 was identified as a novel neuronal interactor of NF-κB p65. In HEK293 cells, a direct physical interaction was shown by co-immunoprecipitation and verified via in situ proximity ligation in healthy rat neurons. Pharmacological blockade of Hsc70 by deoxyspergualin (DSG) strongly decreased nuclear translocation of NF-κB p65 and transcriptional activity shown by reporter gene assays in neurons after stimulation with glutamate. In addition, knock down of Hsc70 via siRNA significantly reduced neuronal NF-κB activity. Taken together these data provide evidence for Hsc70 as a novel neuronal interactor of NF-κB p65. Public Library of Science 2013-06-07 /pmc/articles/PMC3676459/ /pubmed/23762333 http://dx.doi.org/10.1371/journal.pone.0065280 Text en © 2013 Klenke et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Klenke, Christin Widera, Darius Engelen, Thomas Müller, Janine Noll, Thomas Niehaus, Karsten Schmitz, M. Lienhard Kaltschmidt, Barbara Kaltschmidt, Christian
spellingShingle	Klenke, Christin Widera, Darius Engelen, Thomas Müller, Janine Noll, Thomas Niehaus, Karsten Schmitz, M. Lienhard Kaltschmidt, Barbara Kaltschmidt, Christian Hsc70 Is a Novel Interactor of NF-kappaB p65 in Living Hippocampal Neurons
author_facet	Klenke, Christin Widera, Darius Engelen, Thomas Müller, Janine Noll, Thomas Niehaus, Karsten Schmitz, M. Lienhard Kaltschmidt, Barbara Kaltschmidt, Christian
author_sort	Klenke, Christin
title	Hsc70 Is a Novel Interactor of NF-kappaB p65 in Living Hippocampal Neurons
title_short	Hsc70 Is a Novel Interactor of NF-kappaB p65 in Living Hippocampal Neurons
title_full	Hsc70 Is a Novel Interactor of NF-kappaB p65 in Living Hippocampal Neurons
title_fullStr	Hsc70 Is a Novel Interactor of NF-kappaB p65 in Living Hippocampal Neurons
title_full_unstemmed	Hsc70 Is a Novel Interactor of NF-kappaB p65 in Living Hippocampal Neurons
title_sort	hsc70 is a novel interactor of nf-kappab p65 in living hippocampal neurons
description	Signaling via NF-κB in neurons depends on complex formation with interactors such as dynein/dynactin motor complex and can be triggered by synaptic activation. However, so far a detailed interaction map for the neuronal NF-κB is missing. In this study we used mass spectrometry to identify novel interactors of NF-κB p65 within the brain. Hsc70 was identified as a novel neuronal interactor of NF-κB p65. In HEK293 cells, a direct physical interaction was shown by co-immunoprecipitation and verified via in situ proximity ligation in healthy rat neurons. Pharmacological blockade of Hsc70 by deoxyspergualin (DSG) strongly decreased nuclear translocation of NF-κB p65 and transcriptional activity shown by reporter gene assays in neurons after stimulation with glutamate. In addition, knock down of Hsc70 via siRNA significantly reduced neuronal NF-κB activity. Taken together these data provide evidence for Hsc70 as a novel neuronal interactor of NF-κB p65.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3676459/
_version_	1611984746850549760

Hsc70 Is a Novel Interactor of NF-kappaB p65 in Living Hippocampal Neurons

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