Chiral imaging of collagen by second-harmonic generation circular dichroism
We provide evidence that the chirality of collagen can give rise to strong second-harmonic generation circular dichroism (SHG-CD) responses in nonlinear microscopy. Although chirality is an intrinsic structural property of collagen, most of the previous studies ignore that property. We demonstrate c...
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| Format: | Online |
| Language: | English |
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Optical Society of America
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675869/ |
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pubmed-3675869 |
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oai_dc |
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pubmed-36758692013-06-11 Chiral imaging of collagen by second-harmonic generation circular dichroism Lee, H. Huttunen, M. J. Hsu, K.-J. Partanen, M. Zhuo, G.-Y. Kauranen, M. Chu, S.-W. Research-Article We provide evidence that the chirality of collagen can give rise to strong second-harmonic generation circular dichroism (SHG-CD) responses in nonlinear microscopy. Although chirality is an intrinsic structural property of collagen, most of the previous studies ignore that property. We demonstrate chiral imaging of individual collagen fibers by using a laser scanning microscope and type-I collagen from pig ligaments. 100% contrast level of SHG-CD is achieved with sub-micrometer spatial resolution. As a new contrast mechanism for imaging chiral structures in bio-tissues, this technique provides information about collagen morphology and three-dimensional orientation of collagen molecules. Optical Society of America 2013-05-20 /pmc/articles/PMC3675869/ /pubmed/23761852 http://dx.doi.org/10.1364/BOE.4.000909 Text en ©2013 Optical Society of America author-open |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Lee, H. Huttunen, M. J. Hsu, K.-J. Partanen, M. Zhuo, G.-Y. Kauranen, M. Chu, S.-W. |
| spellingShingle |
Lee, H. Huttunen, M. J. Hsu, K.-J. Partanen, M. Zhuo, G.-Y. Kauranen, M. Chu, S.-W. Chiral imaging of collagen by second-harmonic generation circular dichroism |
| author_facet |
Lee, H. Huttunen, M. J. Hsu, K.-J. Partanen, M. Zhuo, G.-Y. Kauranen, M. Chu, S.-W. |
| author_sort |
Lee, H. |
| title |
Chiral imaging of collagen by second-harmonic generation circular dichroism |
| title_short |
Chiral imaging of collagen by second-harmonic generation circular dichroism |
| title_full |
Chiral imaging of collagen by second-harmonic generation circular dichroism |
| title_fullStr |
Chiral imaging of collagen by second-harmonic generation circular dichroism |
| title_full_unstemmed |
Chiral imaging of collagen by second-harmonic generation circular dichroism |
| title_sort |
chiral imaging of collagen by second-harmonic generation circular dichroism |
| description |
We provide evidence that the chirality of collagen can give rise to strong second-harmonic generation circular dichroism (SHG-CD) responses in nonlinear microscopy. Although chirality is an intrinsic structural property of collagen, most of the previous studies ignore that property. We demonstrate chiral imaging of individual collagen fibers by using a laser scanning microscope and type-I collagen from pig ligaments. 100% contrast level of SHG-CD is achieved with sub-micrometer spatial resolution. As a new contrast mechanism for imaging chiral structures in bio-tissues, this technique provides information about collagen morphology and three-dimensional orientation of collagen molecules. |
| publisher |
Optical Society of America |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675869/ |
| _version_ |
1611984668945547264 |