Stable conditional expression and effect of C/EBPβ-LIP in adipocytes using the pSLIK system
Murine 3T3-L1 adipocytes are widely used as a cellular model of obesity. However, whereas transfection of 3T3-L1 preadipocytes is straightforward, ectopic gene expression in mature 3T3-L1 adipocytes has proved challenging. Here, we used the pSLIK vector system to generate stable doxycycline-inducibl...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Society for Endocrinology
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3672996/ |