Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis
Poly-γ-glutamic acid (γ-PGA), naturally secreted from various strains of Bacillus, has anti-inflammatory activity. In inflammatory bowel disease (IBD), inflammation is promoted and sustained by angiogenesis; however, the role played by γ-PGA in this condition is unclear. Therefore, we evaluated γ-PG...
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Hindawi Publishing Corporation
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671540/ |
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pubmed-36715402013-06-13 Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis Davaatseren, Munkhtugs Hwang, Jin-Taek Park, Jae Ho Kim, Myung-Sunny Wang, Shuaiyu Sung, Mi Jeong Research Article Poly-γ-glutamic acid (γ-PGA), naturally secreted from various strains of Bacillus, has anti-inflammatory activity. In inflammatory bowel disease (IBD), inflammation is promoted and sustained by angiogenesis; however, the role played by γ-PGA in this condition is unclear. Therefore, we evaluated γ-PGA effects on angiogenesis and inflammation in a dextran sulfate sodium- (DSS-) induced mouse colitis model. Experimental colitis was induced in male C57BL/6 mice by administering 3% DSS. Disease activity index (DAI), histopathological scores, microvascular density, myeloperoxidase activity, and VEGF-A and VEGFR2 expression were compared among control mice, DSS-treated mice, and mice receiving 3% DSS along with γ-PGA at 50 mg/kg body weight per day or 3% DSS with γ-PGA at 200 mg/kg body weight per day. We found that γ-PGA significantly attenuated weight loss, DAI, and colon shortening. γ-PGA also significantly reduced histopathological evidence of injury. Moreover, γ-PGA significantly attenuated DSS-induced blood vessel densities. Furthermore, γ-PGA attenuated DSS-induced expression of VEGF-A and its receptor, VEGFR2. In addition, γ-PGA treatment led to reduced recruitment of leukocytes to the inflamed colon. Therefore, our results indicate that γ-PGA has potential application in conditions marked by inflammatory-driven angiogenesis and mucosal inflammation. Hindawi Publishing Corporation 2013 2013-05-16 /pmc/articles/PMC3671540/ /pubmed/23766568 http://dx.doi.org/10.1155/2013/982383 Text en Copyright © 2013 Munkhtugs Davaatseren et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Davaatseren, Munkhtugs Hwang, Jin-Taek Park, Jae Ho Kim, Myung-Sunny Wang, Shuaiyu Sung, Mi Jeong |
| spellingShingle |
Davaatseren, Munkhtugs Hwang, Jin-Taek Park, Jae Ho Kim, Myung-Sunny Wang, Shuaiyu Sung, Mi Jeong Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis |
| author_facet |
Davaatseren, Munkhtugs Hwang, Jin-Taek Park, Jae Ho Kim, Myung-Sunny Wang, Shuaiyu Sung, Mi Jeong |
| author_sort |
Davaatseren, Munkhtugs |
| title |
Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis |
| title_short |
Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis |
| title_full |
Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis |
| title_fullStr |
Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis |
| title_full_unstemmed |
Poly-γ-Glutamic Acid Attenuates Angiogenesis and Inflammation in Experimental Colitis |
| title_sort |
poly-γ-glutamic acid attenuates angiogenesis and inflammation in experimental colitis |
| description |
Poly-γ-glutamic acid (γ-PGA), naturally secreted from various strains of Bacillus, has anti-inflammatory activity. In inflammatory bowel disease (IBD), inflammation is promoted and sustained by angiogenesis; however, the role played by γ-PGA in this condition is unclear. Therefore, we evaluated γ-PGA effects on angiogenesis and inflammation in a dextran sulfate sodium- (DSS-) induced mouse colitis model. Experimental colitis was induced in male C57BL/6 mice by administering 3% DSS. Disease activity index (DAI), histopathological scores, microvascular density, myeloperoxidase activity, and VEGF-A and VEGFR2 expression were compared among control mice, DSS-treated mice, and mice receiving 3% DSS along with γ-PGA at 50 mg/kg body weight per day or 3% DSS with γ-PGA at 200 mg/kg body weight per day. We found that γ-PGA significantly attenuated weight loss, DAI, and colon shortening. γ-PGA also significantly reduced histopathological evidence of injury. Moreover, γ-PGA significantly attenuated DSS-induced blood vessel densities. Furthermore, γ-PGA attenuated DSS-induced expression of VEGF-A and its receptor, VEGFR2. In addition, γ-PGA treatment led to reduced recruitment of leukocytes to the inflamed colon. Therefore, our results indicate that γ-PGA has potential application in conditions marked by inflammatory-driven angiogenesis and mucosal inflammation. |
| publisher |
Hindawi Publishing Corporation |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3671540/ |
| _version_ |
1611983358114398208 |