Fusing a Carbohydrate-Binding Module into the Aspergillus usamii β-Mannanase to Improve Its Thermostability and Cellulose-Binding Capacity by In Silico Design

The AuMan5A, an acidophilic glycoside hydrolase (GH) family 5 β-mannanase derived from Aspergillus usamii YL-01-78, consists of an only catalytic domain (CD). To perfect enzymatic properties of the AuMan5A, a family 1 carbohydrate-binding module (CBM) of the Trichoderma reesei cellobiohydrolase I (T...

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Bibliographic Details
Main Authors: Tang, Cun-Duo, Li, Jian-Fang, Wei, Xi-Huan, Min, Rou, Gao, Shu-Juan, Wang, Jun-Qing, Yin, Xin, Wu, Min-Chen
Format: Online
Language:English
Published: Public Library of Science 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669383/