Serial Passaging of Candida albicans in Systemic Murine Infection Suggests That the Wild Type Strain SC5314 Is Well Adapted to the Murine Kidney
The opportunistic fungal pathogen Candida albicans has a remarkable ability to adapt to unfavorable environments by different mechanisms, including microevolution. For example, a previous study has shown that passaging through the murine spleen can cause new phenotypic characteristics. Since the mur...
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Public Library of Science
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3667833/ |
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pubmed-36678332013-06-04 Serial Passaging of Candida albicans in Systemic Murine Infection Suggests That the Wild Type Strain SC5314 Is Well Adapted to the Murine Kidney Lüttich, Anja Brunke, Sascha Hube, Bernhard Jacobsen, Ilse D. Research Article The opportunistic fungal pathogen Candida albicans has a remarkable ability to adapt to unfavorable environments by different mechanisms, including microevolution. For example, a previous study has shown that passaging through the murine spleen can cause new phenotypic characteristics. Since the murine kidney is the main target organ in murine Candida sepsis and infection of the spleen differs from the kidney in several aspects, we tested whether C. albicans SC5314 could evolve to further adapt to infection and persistence within the kidney. Therefore, we performed a long-term serial passage experiment through the murine kidney of using a low infectious dose. We found that the overall virulence of the commonly used wild type strain SC5314 did not change after eight passages and that the isolated pools showed only very moderate changes of phenotypic traits on the population level. Nevertheless, the last passage showed a higher phenotypic variability and a few individual strains exhibited phenotypic alterations suggesting that microevolution has occurred. However, the majority of the tested single strains were phenotypically indistinguishable from SC5314. Thus, our findings indicate that characteristics of SC5314 which are important to establish and maintain kidney infection over a prolonged time are already well developed. Public Library of Science 2013-05-30 /pmc/articles/PMC3667833/ /pubmed/23737985 http://dx.doi.org/10.1371/journal.pone.0064482 Text en © 2013 Lüttich et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Lüttich, Anja Brunke, Sascha Hube, Bernhard Jacobsen, Ilse D. |
| spellingShingle |
Lüttich, Anja Brunke, Sascha Hube, Bernhard Jacobsen, Ilse D. Serial Passaging of Candida albicans in Systemic Murine Infection Suggests That the Wild Type Strain SC5314 Is Well Adapted to the Murine Kidney |
| author_facet |
Lüttich, Anja Brunke, Sascha Hube, Bernhard Jacobsen, Ilse D. |
| author_sort |
Lüttich, Anja |
| title |
Serial Passaging of Candida albicans in Systemic Murine Infection Suggests That the Wild Type Strain SC5314 Is Well Adapted to the Murine Kidney |
| title_short |
Serial Passaging of Candida albicans in Systemic Murine Infection Suggests That the Wild Type Strain SC5314 Is Well Adapted to the Murine Kidney |
| title_full |
Serial Passaging of Candida albicans in Systemic Murine Infection Suggests That the Wild Type Strain SC5314 Is Well Adapted to the Murine Kidney |
| title_fullStr |
Serial Passaging of Candida albicans in Systemic Murine Infection Suggests That the Wild Type Strain SC5314 Is Well Adapted to the Murine Kidney |
| title_full_unstemmed |
Serial Passaging of Candida albicans in Systemic Murine Infection Suggests That the Wild Type Strain SC5314 Is Well Adapted to the Murine Kidney |
| title_sort |
serial passaging of candida albicans in systemic murine infection suggests that the wild type strain sc5314 is well adapted to the murine kidney |
| description |
The opportunistic fungal pathogen Candida albicans has a remarkable ability to adapt to unfavorable environments by different mechanisms, including microevolution. For example, a previous study has shown that passaging through the murine spleen can cause new phenotypic characteristics. Since the murine kidney is the main target organ in murine Candida sepsis and infection of the spleen differs from the kidney in several aspects, we tested whether C. albicans SC5314 could evolve to further adapt to infection and persistence within the kidney. Therefore, we performed a long-term serial passage experiment through the murine kidney of using a low infectious dose. We found that the overall virulence of the commonly used wild type strain SC5314 did not change after eight passages and that the isolated pools showed only very moderate changes of phenotypic traits on the population level. Nevertheless, the last passage showed a higher phenotypic variability and a few individual strains exhibited phenotypic alterations suggesting that microevolution has occurred. However, the majority of the tested single strains were phenotypically indistinguishable from SC5314. Thus, our findings indicate that characteristics of SC5314 which are important to establish and maintain kidney infection over a prolonged time are already well developed. |
| publisher |
Public Library of Science |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3667833/ |
| _version_ |
1611982255726526464 |