Mucosal Barrier in Ulcerative Colitis and Crohn's Disease

Background. The mucus layer in the gastrointestinal tract plays important role in host innate defense, regulation of secretion, and absorption processes, maintaining colonization resistance, which composes the integrity of protective mucus barrier in the large intestine. Investigations of mucin exp...

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Main Authors:	Dorofeyev, A. E., Vasilenko, I. V., Rassokhina, O. A., Kondratiuk, R. B.
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664489/

id	pubmed-3664489
recordtype	oai_dc
spelling	pubmed-36644892013-06-04 Mucosal Barrier in Ulcerative Colitis and Crohn's Disease Dorofeyev, A. E. Vasilenko, I. V. Rassokhina, O. A. Kondratiuk, R. B. Clinical Study Background. The mucus layer in the gastrointestinal tract plays important role in host innate defense, regulation of secretion, and absorption processes, maintaining colonization resistance, which composes the integrity of protective mucus barrier in the large intestine. Investigations of mucin expression in the colon mucosa can improve the understanding of protective function of mucosal barrier in ulcerative colitis (UC) and Crohn's disease (CD). Materials and Methods. 77 patients with UC and CD were examined. Histological analysis of colon mucosa was done by standard method (haematoxylin-eosin, alcian blue at pH 1.0 and 2.5 to determine sulfated and nonsulfated glycosaminoglycans and glycoproteins, and goblet cells). To characterize the mucus production the PAS-reaction was performed. Immunohistochemistry was performed using monoclonal mouse antibodies raised against MUC2, MUC3, MUC4, and TFF3 (USBiological, USA). Results. The moderate expression of MUC2 and MUC3 (50.0% and 32.1%, P = 0.03) and high expression of MUC4 and TFF3 in the colon mucosa were observed in all patients with CD. The intensive labeling of MUC4 and TFF3 occurred more often (42.9% and 57.1%, P = 0.03) in patients with CD. The level of expression of secretory MUC2 and transmembrane MUC3 and MUC4 in all patients with UC was low, up to its complete absence (59.2% and 53.1% cases, P = 0.05). TFF3 expression had high and medium staining intensity in patients with UC. Conclusions. Different types of mucins synthesis, secretion, and expression were found in patients with UC and CD. The expression of mucin MUC2, MUC3, MUC4, and TFF3 correlated with the activity of disease and the extent of the inflammatory process in the large intestine. The most pronounced alteration of mucins expression was observed in patients with severe UC and CD. Hindawi Publishing Corporation 2013 2013-05-07 /pmc/articles/PMC3664489/ /pubmed/23737764 http://dx.doi.org/10.1155/2013/431231 Text en Copyright © 2013 A. E. Dorofeyev et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Dorofeyev, A. E. Vasilenko, I. V. Rassokhina, O. A. Kondratiuk, R. B.
spellingShingle	Dorofeyev, A. E. Vasilenko, I. V. Rassokhina, O. A. Kondratiuk, R. B. Mucosal Barrier in Ulcerative Colitis and Crohn's Disease
author_facet	Dorofeyev, A. E. Vasilenko, I. V. Rassokhina, O. A. Kondratiuk, R. B.
author_sort	Dorofeyev, A. E.
title	Mucosal Barrier in Ulcerative Colitis and Crohn's Disease
title_short	Mucosal Barrier in Ulcerative Colitis and Crohn's Disease
title_full	Mucosal Barrier in Ulcerative Colitis and Crohn's Disease
title_fullStr	Mucosal Barrier in Ulcerative Colitis and Crohn's Disease
title_full_unstemmed	Mucosal Barrier in Ulcerative Colitis and Crohn's Disease
title_sort	mucosal barrier in ulcerative colitis and crohn's disease
description	Background. The mucus layer in the gastrointestinal tract plays important role in host innate defense, regulation of secretion, and absorption processes, maintaining colonization resistance, which composes the integrity of protective mucus barrier in the large intestine. Investigations of mucin expression in the colon mucosa can improve the understanding of protective function of mucosal barrier in ulcerative colitis (UC) and Crohn's disease (CD). Materials and Methods. 77 patients with UC and CD were examined. Histological analysis of colon mucosa was done by standard method (haematoxylin-eosin, alcian blue at pH 1.0 and 2.5 to determine sulfated and nonsulfated glycosaminoglycans and glycoproteins, and goblet cells). To characterize the mucus production the PAS-reaction was performed. Immunohistochemistry was performed using monoclonal mouse antibodies raised against MUC2, MUC3, MUC4, and TFF3 (USBiological, USA). Results. The moderate expression of MUC2 and MUC3 (50.0% and 32.1%, P = 0.03) and high expression of MUC4 and TFF3 in the colon mucosa were observed in all patients with CD. The intensive labeling of MUC4 and TFF3 occurred more often (42.9% and 57.1%, P = 0.03) in patients with CD. The level of expression of secretory MUC2 and transmembrane MUC3 and MUC4 in all patients with UC was low, up to its complete absence (59.2% and 53.1% cases, P = 0.05). TFF3 expression had high and medium staining intensity in patients with UC. Conclusions. Different types of mucins synthesis, secretion, and expression were found in patients with UC and CD. The expression of mucin MUC2, MUC3, MUC4, and TFF3 correlated with the activity of disease and the extent of the inflammatory process in the large intestine. The most pronounced alteration of mucins expression was observed in patients with severe UC and CD.
publisher	Hindawi Publishing Corporation
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664489/
_version_	1611981207081320448

Mucosal Barrier in Ulcerative Colitis and Crohn's Disease

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