Biological N2O Fixation in the Eastern South Pacific Ocean and Marine Cyanobacterial Cultures

Despite the importance of nitrous oxide (N2O) in the global radiative balance and atmospheric ozone chemistry, its sources and sinks within the Earth’s system are still poorly understood. In the ocean, N2O is produced by microbiological processes such as nitrification and partial denitrification, wh...

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Main Authors:	Farías, Laura, Faúndez, Juan, Fernández, Camila, Cornejo, Marcela, Sanhueza, Sandra, Carrasco, Cristina
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662754/

id	pubmed-3662754
recordtype	oai_dc
spelling	pubmed-36627542013-05-28 Biological N2O Fixation in the Eastern South Pacific Ocean and Marine Cyanobacterial Cultures Farías, Laura Faúndez, Juan Fernández, Camila Cornejo, Marcela Sanhueza, Sandra Carrasco, Cristina Research Article Despite the importance of nitrous oxide (N2O) in the global radiative balance and atmospheric ozone chemistry, its sources and sinks within the Earth’s system are still poorly understood. In the ocean, N2O is produced by microbiological processes such as nitrification and partial denitrification, which account for about a third of global emissions. Conversely, complete denitrification (the dissimilative reduction of N2O to N2) under suboxic/anoxic conditions is the only known pathway accountable for N2O consumption in the ocean. In this work, it is demonstrated that the biological assimilation of N2O could be a significant pathway capable of directly transforming this gas into particulate organic nitrogen (PON). N2O is shown to be biologically fixed within the subtropical and tropical waters of the eastern South Pacific Ocean, under a wide range of oceanographic conditions and at rates ranging from 2 pmol N L−1 d− to 14.8 nmol N L−1 d−1 (mean ± SE of 0.522±1.06 nmol N L−1 d−1, n = 93). Additional assays revealed that cultured cyanobacterial strains of Trichodesmium (H-9 and IMS 101), and Crocosphaera (W-8501) have the capacity to directly fix N2O under laboratory conditions; suggesting that marine photoautotrophic diazotrophs could be using N2O as a substrate. This metabolic capacity however was absent in Synechococcus (RCC 1029). The findings presented here indicate that assimilative N2O fixation takes place under extreme environmental conditions (i.e., light, nutrient, oxygen) where both autotrophic (including cyanobacteria) and heterotrophic microbes appear to be involved. This process could provide a globally significant sink for atmospheric N2O which in turn affects the oceanic N2O inventory and may also represent a yet unexplored global oceanic source of fixed N. Public Library of Science 2013-05-23 /pmc/articles/PMC3662754/ /pubmed/23717516 http://dx.doi.org/10.1371/journal.pone.0063956 Text en © 2013 Farías et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Farías, Laura Faúndez, Juan Fernández, Camila Cornejo, Marcela Sanhueza, Sandra Carrasco, Cristina
spellingShingle	Farías, Laura Faúndez, Juan Fernández, Camila Cornejo, Marcela Sanhueza, Sandra Carrasco, Cristina Biological N2O Fixation in the Eastern South Pacific Ocean and Marine Cyanobacterial Cultures
author_facet	Farías, Laura Faúndez, Juan Fernández, Camila Cornejo, Marcela Sanhueza, Sandra Carrasco, Cristina
author_sort	Farías, Laura
title	Biological N2O Fixation in the Eastern South Pacific Ocean and Marine Cyanobacterial Cultures
title_short	Biological N2O Fixation in the Eastern South Pacific Ocean and Marine Cyanobacterial Cultures
title_full	Biological N2O Fixation in the Eastern South Pacific Ocean and Marine Cyanobacterial Cultures
title_fullStr	Biological N2O Fixation in the Eastern South Pacific Ocean and Marine Cyanobacterial Cultures
title_full_unstemmed	Biological N2O Fixation in the Eastern South Pacific Ocean and Marine Cyanobacterial Cultures
title_sort	biological n2o fixation in the eastern south pacific ocean and marine cyanobacterial cultures
description	Despite the importance of nitrous oxide (N2O) in the global radiative balance and atmospheric ozone chemistry, its sources and sinks within the Earth’s system are still poorly understood. In the ocean, N2O is produced by microbiological processes such as nitrification and partial denitrification, which account for about a third of global emissions. Conversely, complete denitrification (the dissimilative reduction of N2O to N2) under suboxic/anoxic conditions is the only known pathway accountable for N2O consumption in the ocean. In this work, it is demonstrated that the biological assimilation of N2O could be a significant pathway capable of directly transforming this gas into particulate organic nitrogen (PON). N2O is shown to be biologically fixed within the subtropical and tropical waters of the eastern South Pacific Ocean, under a wide range of oceanographic conditions and at rates ranging from 2 pmol N L−1 d− to 14.8 nmol N L−1 d−1 (mean ± SE of 0.522±1.06 nmol N L−1 d−1, n = 93). Additional assays revealed that cultured cyanobacterial strains of Trichodesmium (H-9 and IMS 101), and Crocosphaera (W-8501) have the capacity to directly fix N2O under laboratory conditions; suggesting that marine photoautotrophic diazotrophs could be using N2O as a substrate. This metabolic capacity however was absent in Synechococcus (RCC 1029). The findings presented here indicate that assimilative N2O fixation takes place under extreme environmental conditions (i.e., light, nutrient, oxygen) where both autotrophic (including cyanobacteria) and heterotrophic microbes appear to be involved. This process could provide a globally significant sink for atmospheric N2O which in turn affects the oceanic N2O inventory and may also represent a yet unexplored global oceanic source of fixed N.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662754/
_version_	1611980656658612224

Biological N2O Fixation in the Eastern South Pacific Ocean and Marine Cyanobacterial Cultures

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