Restoration of Sensitivity in Chemo — Resistant Glioma Cells by Cold Atmospheric Plasma

Glioblastoma (GBM) is the most common and aggressive brain tumor in adults. Despite multimodal treatments including surgery, chemotherapy and radiotherapy the prognosis remains poor and relapse occurs regularly. The alkylating agent temozolomide (TMZ) has been shown to improve the overall survival i...

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Main Authors:	Köritzer, Julia, Boxhammer, Veronika, Schäfer, Andrea, Shimizu, Tetsuji, Klämpfl, Tobias G., Li, Yang-Fang, Welz, Christian, Schwenk-Zieger, Sabina, Morfill, Gregor E., Zimmermann, Julia L., Schlegel, Jürgen
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660344/

id	pubmed-3660344
recordtype	oai_dc
spelling	pubmed-36603442013-05-23 Restoration of Sensitivity in Chemo — Resistant Glioma Cells by Cold Atmospheric Plasma Köritzer, Julia Boxhammer, Veronika Schäfer, Andrea Shimizu, Tetsuji Klämpfl, Tobias G. Li, Yang-Fang Welz, Christian Schwenk-Zieger, Sabina Morfill, Gregor E. Zimmermann, Julia L. Schlegel, Jürgen Research Article Glioblastoma (GBM) is the most common and aggressive brain tumor in adults. Despite multimodal treatments including surgery, chemotherapy and radiotherapy the prognosis remains poor and relapse occurs regularly. The alkylating agent temozolomide (TMZ) has been shown to improve the overall survival in patients with malignant gliomas, especially in tumors with methylated promoter of the O6-methylguanine-DNA-methyltransferase (MGMT) gene. However, intrinsic and acquired resistance towards TMZ makes it crucial to find new therapeutic strategies aimed at improving the prognosis of patients suffering from malignant gliomas. Cold atmospheric plasma is a new auspicious candidate in cancer treatment. In the present study we demonstrate the anti-cancer properties of different dosages of cold atmospheric plasma (CAP) both in TMZ-sensitive and TMZ-resistant cells by proliferation assay, immunoblotting, cell cycle analysis, and clonogenicity assay. Importantly, CAP treatment restored the responsiveness of resistant glioma cells towards TMZ therapy. Concomitant treatment with CAP and TMZ led to inhibition of cell growth and cell cycle arrest, thus CAP might be a promising candidate for combination therapy especially for patients suffering from GBMs showing an unfavorable MGMT status and TMZ resistance. Public Library of Science 2013-05-21 /pmc/articles/PMC3660344/ /pubmed/23704990 http://dx.doi.org/10.1371/journal.pone.0064498 Text en © 2013 Köritzer et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Köritzer, Julia Boxhammer, Veronika Schäfer, Andrea Shimizu, Tetsuji Klämpfl, Tobias G. Li, Yang-Fang Welz, Christian Schwenk-Zieger, Sabina Morfill, Gregor E. Zimmermann, Julia L. Schlegel, Jürgen
spellingShingle	Köritzer, Julia Boxhammer, Veronika Schäfer, Andrea Shimizu, Tetsuji Klämpfl, Tobias G. Li, Yang-Fang Welz, Christian Schwenk-Zieger, Sabina Morfill, Gregor E. Zimmermann, Julia L. Schlegel, Jürgen Restoration of Sensitivity in Chemo — Resistant Glioma Cells by Cold Atmospheric Plasma
author_facet	Köritzer, Julia Boxhammer, Veronika Schäfer, Andrea Shimizu, Tetsuji Klämpfl, Tobias G. Li, Yang-Fang Welz, Christian Schwenk-Zieger, Sabina Morfill, Gregor E. Zimmermann, Julia L. Schlegel, Jürgen
author_sort	Köritzer, Julia
title	Restoration of Sensitivity in Chemo — Resistant Glioma Cells by Cold Atmospheric Plasma
title_short	Restoration of Sensitivity in Chemo — Resistant Glioma Cells by Cold Atmospheric Plasma
title_full	Restoration of Sensitivity in Chemo — Resistant Glioma Cells by Cold Atmospheric Plasma
title_fullStr	Restoration of Sensitivity in Chemo — Resistant Glioma Cells by Cold Atmospheric Plasma
title_full_unstemmed	Restoration of Sensitivity in Chemo — Resistant Glioma Cells by Cold Atmospheric Plasma
title_sort	restoration of sensitivity in chemo — resistant glioma cells by cold atmospheric plasma
description	Glioblastoma (GBM) is the most common and aggressive brain tumor in adults. Despite multimodal treatments including surgery, chemotherapy and radiotherapy the prognosis remains poor and relapse occurs regularly. The alkylating agent temozolomide (TMZ) has been shown to improve the overall survival in patients with malignant gliomas, especially in tumors with methylated promoter of the O6-methylguanine-DNA-methyltransferase (MGMT) gene. However, intrinsic and acquired resistance towards TMZ makes it crucial to find new therapeutic strategies aimed at improving the prognosis of patients suffering from malignant gliomas. Cold atmospheric plasma is a new auspicious candidate in cancer treatment. In the present study we demonstrate the anti-cancer properties of different dosages of cold atmospheric plasma (CAP) both in TMZ-sensitive and TMZ-resistant cells by proliferation assay, immunoblotting, cell cycle analysis, and clonogenicity assay. Importantly, CAP treatment restored the responsiveness of resistant glioma cells towards TMZ therapy. Concomitant treatment with CAP and TMZ led to inhibition of cell growth and cell cycle arrest, thus CAP might be a promising candidate for combination therapy especially for patients suffering from GBMs showing an unfavorable MGMT status and TMZ resistance.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660344/
_version_	1611979838000726016

Restoration of Sensitivity in Chemo — Resistant Glioma Cells by Cold Atmospheric Plasma

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