An Edible Gintonin Preparation from Ginseng

Ginseng, the root of Panax ginseng, is one of the oldest herbal medicines. It has a variety of physiological and pharmacological effects. Recently, we isolated a subset of glycolipoproteins that we designated gintonin, and demonstrated that it induced transient change in intracellular calcium concen...

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Main Authors:	Choi, Sun-Hye, Shin, Tae-Joon, Lee, Byung-Hwan, Hwang, Sung Hee, Kang, Jiyeon, Kim, Hyun-Joong, Park, Chan-Woo, Nah, Seung-Yeol
Format:	Online
Language:	English
Published:	The Korean Society of Ginseng 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659555/

id	pubmed-3659555
recordtype	oai_dc
spelling	pubmed-36595552013-05-28 An Edible Gintonin Preparation from Ginseng Choi, Sun-Hye Shin, Tae-Joon Lee, Byung-Hwan Hwang, Sung Hee Kang, Jiyeon Kim, Hyun-Joong Park, Chan-Woo Nah, Seung-Yeol Articles Ginseng, the root of Panax ginseng, is one of the oldest herbal medicines. It has a variety of physiological and pharmacological effects. Recently, we isolated a subset of glycolipoproteins that we designated gintonin, and demonstrated that it induced transient change in intracellular calcium concentration ([Ca2+]i) in cells via G-protein-coupled receptor signaling pathway(s). The previous method for gintonin isolation included multiple steps using methanol, butanol, and other organic solvents. In the present study, we developed a much simple method for the preparation of gintonin from ginseng root using 80% ethanol extraction. The extracted fraction was designated edible gintonin. This method produced a high yield of gintonin (0.20%). The chemical characteristics of gintonin such as molecular weight and the composition of the extract product were almost identical as the gintonin prepared using the previous extraction regimen involving various organic solvents. We also examined the physiological effects of edible gintonin on endogenous Ca2+-activated Cl- channel activity of Xenopus oocytes. The 50% effective dose was 1.03±0.3 μg/mL. Finally, since gintonin preparation through ethanol extraction is easily reproducible, gintonin could be commercially applied for ginseng-derived functional health food and/or drug following the confirmations of in vitro and in vivo physiological and pharmacological effects of gintonin. The Korean Society of Ginseng 2011-11 /pmc/articles/PMC3659555/ /pubmed/23717094 http://dx.doi.org/10.5142/jgr.2011.35.4.471 Text en Copyright ©2011, The Korean Society of Ginseng http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Choi, Sun-Hye Shin, Tae-Joon Lee, Byung-Hwan Hwang, Sung Hee Kang, Jiyeon Kim, Hyun-Joong Park, Chan-Woo Nah, Seung-Yeol
spellingShingle	Choi, Sun-Hye Shin, Tae-Joon Lee, Byung-Hwan Hwang, Sung Hee Kang, Jiyeon Kim, Hyun-Joong Park, Chan-Woo Nah, Seung-Yeol An Edible Gintonin Preparation from Ginseng
author_facet	Choi, Sun-Hye Shin, Tae-Joon Lee, Byung-Hwan Hwang, Sung Hee Kang, Jiyeon Kim, Hyun-Joong Park, Chan-Woo Nah, Seung-Yeol
author_sort	Choi, Sun-Hye
title	An Edible Gintonin Preparation from Ginseng
title_short	An Edible Gintonin Preparation from Ginseng
title_full	An Edible Gintonin Preparation from Ginseng
title_fullStr	An Edible Gintonin Preparation from Ginseng
title_full_unstemmed	An Edible Gintonin Preparation from Ginseng
title_sort	edible gintonin preparation from ginseng
description	Ginseng, the root of Panax ginseng, is one of the oldest herbal medicines. It has a variety of physiological and pharmacological effects. Recently, we isolated a subset of glycolipoproteins that we designated gintonin, and demonstrated that it induced transient change in intracellular calcium concentration ([Ca2+]i) in cells via G-protein-coupled receptor signaling pathway(s). The previous method for gintonin isolation included multiple steps using methanol, butanol, and other organic solvents. In the present study, we developed a much simple method for the preparation of gintonin from ginseng root using 80% ethanol extraction. The extracted fraction was designated edible gintonin. This method produced a high yield of gintonin (0.20%). The chemical characteristics of gintonin such as molecular weight and the composition of the extract product were almost identical as the gintonin prepared using the previous extraction regimen involving various organic solvents. We also examined the physiological effects of edible gintonin on endogenous Ca2+-activated Cl- channel activity of Xenopus oocytes. The 50% effective dose was 1.03±0.3 μg/mL. Finally, since gintonin preparation through ethanol extraction is easily reproducible, gintonin could be commercially applied for ginseng-derived functional health food and/or drug following the confirmations of in vitro and in vivo physiological and pharmacological effects of gintonin.
publisher	The Korean Society of Ginseng
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659555/
_version_	1611979627355439104

An Edible Gintonin Preparation from Ginseng

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