Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis
Suid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky's disease. The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV. The aim of this study was to standardize a rapid method for genotypi...
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| Format: | Online |
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International Scholarly Research Network
2011
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658827/ |
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pubmed-3658827 |
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pubmed-36588272013-05-30 Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis Fonseca, Antônio Augusto Magalhães, Cristina Gonçalves Sales, Érica Bravo D'Ambros, Régia Maria Ciacci-Zanella, Janice Heinemann, Marcos Bryan Leite, Rômulo Cerqueira dos Reis, Jenner Karlisson Pimenta Research Article Suid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky's disease. The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV. The aim of this study was to standardize a rapid method for genotyping SuHV-1 without virus isolation, using a multiplex-PCR followed by enzymatic restriction analysis. The complete genome of the virus was analyzed in silico to determine the restriction sites for the enzyme BamHI. Primers were designed to flank sites with emphasis on certain points of differentiation of genotypes. The standard PCRs were able to detect the SuHV-1 and also to differentiate genotypes from brain tissue of infected pigs. The BamHI-PCR is a rapid, practical, and sensitive way to genotype SuHV-1. International Scholarly Research Network 2011-11-24 /pmc/articles/PMC3658827/ /pubmed/23724304 http://dx.doi.org/10.5402/2011/458294 Text en Copyright © 2011 Antônio Augusto Fonseca Jr. et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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Open Access Journal |
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Foreign Institution |
| institution |
US National Center for Biotechnology Information |
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NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Fonseca, Antônio Augusto Magalhães, Cristina Gonçalves Sales, Érica Bravo D'Ambros, Régia Maria Ciacci-Zanella, Janice Heinemann, Marcos Bryan Leite, Rômulo Cerqueira dos Reis, Jenner Karlisson Pimenta |
| spellingShingle |
Fonseca, Antônio Augusto Magalhães, Cristina Gonçalves Sales, Érica Bravo D'Ambros, Régia Maria Ciacci-Zanella, Janice Heinemann, Marcos Bryan Leite, Rômulo Cerqueira dos Reis, Jenner Karlisson Pimenta Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis |
| author_facet |
Fonseca, Antônio Augusto Magalhães, Cristina Gonçalves Sales, Érica Bravo D'Ambros, Régia Maria Ciacci-Zanella, Janice Heinemann, Marcos Bryan Leite, Rômulo Cerqueira dos Reis, Jenner Karlisson Pimenta |
| author_sort |
Fonseca, Antônio Augusto |
| title |
Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis |
| title_short |
Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis |
| title_full |
Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis |
| title_fullStr |
Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis |
| title_full_unstemmed |
Genotyping of the Pseudorabies Virus by Multiplex PCR Followed by Restriction Enzyme Analysis |
| title_sort |
genotyping of the pseudorabies virus by multiplex pcr followed by restriction enzyme analysis |
| description |
Suid herpesvirus 1 (SuHV-1) is the causative agent of Aujeszky's disease. The infectious agent has only one serotype, but it was classified by restriction enzyme analysis of the whole genome into four genotypes, named I to IV. The aim of this study was to standardize a rapid method for genotyping SuHV-1 without virus isolation, using a multiplex-PCR followed by enzymatic restriction analysis. The complete genome of the virus was analyzed in silico to determine the restriction sites for the enzyme BamHI. Primers were designed to flank sites with emphasis on certain points of differentiation of genotypes. The standard PCRs were able to detect the SuHV-1 and also to differentiate genotypes from brain tissue of infected pigs. The BamHI-PCR is a rapid, practical, and sensitive way to genotype SuHV-1. |
| publisher |
International Scholarly Research Network |
| publishDate |
2011 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658827/ |
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1611979357056663552 |