Defining ELISpot cut-offs from unreplicated test and control wells
In the absence of replication of wells, empirical criteria for enzyme-linked immunospot (ELISpot) positivity use fixed differences or ratios between spot forming units (SFU) counts between test and control. We propose an alternative approach which first identifies the optimally variance-stabilizing...
Main Authors: | , , , , , , , |
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Format: | Online |
Language: | English |
Published: |
Elsevier
2013
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Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657161/ |