Defining ELISpot cut-offs from unreplicated test and control wells

In the absence of replication of wells, empirical criteria for enzyme-linked immunospot (ELISpot) positivity use fixed differences or ratios between spot forming units (SFU) counts between test and control. We propose an alternative approach which first identifies the optimally variance-stabilizing...

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Bibliographic Details
Main Authors: Alexander, Neal, Fox, Annette, Lien, Vu Thi Kim, Dong, Tao, Lee, Laurel Yong-Hwa, Le Khanh Hang, Nguyen, Mai, Le Quynh, Horby, Peter
Format: Online
Language:English
Published: Elsevier 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657161/