GAPDH Pseudogenes and the Quantification of Feline Genomic DNA Equivalents

Quantitative real-time PCR (qPCR) is broadly used to detect and quantify nucleic acid targets. In order to determine cell copy number and genome equivalents, a suitable reference gene that is present in a defined number in the genome is needed, preferably as a single copy gene. For most organisms, a...

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Bibliographic Details
Main Authors: Helfer-Hungerbuehler, A. Katrin, Widmer, Stefan, Hofmann-Lehmann, Regina
Format: Online
Language:English
Published: Hindawi Publishing Corporation 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655645/