Quantitative Proteomic Analysis of Cell Cycle of the Dinoflagellate Prorocentrum donghaiense (Dinophyceae)

Dinoflagellates are the major causative agents of harmful algal blooms in the coastal zone, which has resulted in adverse effects on the marine ecosystem and public health, and has become a global concern. Knowledge of cell cycle regulation in proliferating cells is essential for understanding bloom...

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Main Authors:	Wang, Da-Zhi, Zhang, Ying-Jiao, Zhang, Shu-Fei, Lin, Lin, Hong, Hua-Sheng
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655175/

id	pubmed-3655175
recordtype	oai_dc
spelling	pubmed-36551752013-05-20 Quantitative Proteomic Analysis of Cell Cycle of the Dinoflagellate Prorocentrum donghaiense (Dinophyceae) Wang, Da-Zhi Zhang, Ying-Jiao Zhang, Shu-Fei Lin, Lin Hong, Hua-Sheng Research Article Dinoflagellates are the major causative agents of harmful algal blooms in the coastal zone, which has resulted in adverse effects on the marine ecosystem and public health, and has become a global concern. Knowledge of cell cycle regulation in proliferating cells is essential for understanding bloom dynamics, and so this study compared the protein profiles of Prorocentrum donghaiense at different cell cycle phases and identified differentially expressed proteins using 2-D fluorescence difference gel electrophoresis combined with MALDI-TOF-TOF mass spectrometry. The results showed that the synchronized cells of P. donghaiense completed a cell cycle within 24 hours and cell division was phased with the diurnal cycle. Comparison of the protein profiles at four cell cycle phases (G1, S, early and late G2/M) showed that 53 protein spots altered significantly in abundance. Among them, 41 were identified to be involved in a variety of biological processes, e.g. cell cycle and division, RNA metabolism, protein and amino acid metabolism, energy and carbon metabolism, oxidation-reduction processes, and ABC transport. The periodic expression of these proteins was critical to maintain the proper order and function of the cell cycle. This study, to our knowledge, for the first time revealed the major biological processes occurring at different cell cycle phases which provided new insights into the mechanisms regulating the cell cycle and growth of dinoflagellates. Public Library of Science 2013-05-15 /pmc/articles/PMC3655175/ /pubmed/23691081 http://dx.doi.org/10.1371/journal.pone.0063659 Text en © 2013 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Wang, Da-Zhi Zhang, Ying-Jiao Zhang, Shu-Fei Lin, Lin Hong, Hua-Sheng
spellingShingle	Wang, Da-Zhi Zhang, Ying-Jiao Zhang, Shu-Fei Lin, Lin Hong, Hua-Sheng Quantitative Proteomic Analysis of Cell Cycle of the Dinoflagellate Prorocentrum donghaiense (Dinophyceae)
author_facet	Wang, Da-Zhi Zhang, Ying-Jiao Zhang, Shu-Fei Lin, Lin Hong, Hua-Sheng
author_sort	Wang, Da-Zhi
title	Quantitative Proteomic Analysis of Cell Cycle of the Dinoflagellate Prorocentrum donghaiense (Dinophyceae)
title_short	Quantitative Proteomic Analysis of Cell Cycle of the Dinoflagellate Prorocentrum donghaiense (Dinophyceae)
title_full	Quantitative Proteomic Analysis of Cell Cycle of the Dinoflagellate Prorocentrum donghaiense (Dinophyceae)
title_fullStr	Quantitative Proteomic Analysis of Cell Cycle of the Dinoflagellate Prorocentrum donghaiense (Dinophyceae)
title_full_unstemmed	Quantitative Proteomic Analysis of Cell Cycle of the Dinoflagellate Prorocentrum donghaiense (Dinophyceae)
title_sort	quantitative proteomic analysis of cell cycle of the dinoflagellate prorocentrum donghaiense (dinophyceae)
description	Dinoflagellates are the major causative agents of harmful algal blooms in the coastal zone, which has resulted in adverse effects on the marine ecosystem and public health, and has become a global concern. Knowledge of cell cycle regulation in proliferating cells is essential for understanding bloom dynamics, and so this study compared the protein profiles of Prorocentrum donghaiense at different cell cycle phases and identified differentially expressed proteins using 2-D fluorescence difference gel electrophoresis combined with MALDI-TOF-TOF mass spectrometry. The results showed that the synchronized cells of P. donghaiense completed a cell cycle within 24 hours and cell division was phased with the diurnal cycle. Comparison of the protein profiles at four cell cycle phases (G1, S, early and late G2/M) showed that 53 protein spots altered significantly in abundance. Among them, 41 were identified to be involved in a variety of biological processes, e.g. cell cycle and division, RNA metabolism, protein and amino acid metabolism, energy and carbon metabolism, oxidation-reduction processes, and ABC transport. The periodic expression of these proteins was critical to maintain the proper order and function of the cell cycle. This study, to our knowledge, for the first time revealed the major biological processes occurring at different cell cycle phases which provided new insights into the mechanisms regulating the cell cycle and growth of dinoflagellates.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3655175/
_version_	1611978144905953280

Quantitative Proteomic Analysis of Cell Cycle of the Dinoflagellate Prorocentrum donghaiense (Dinophyceae)

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