Nonlinear microscopy of eumelanin and pheomelanin with sub-cellular resolution

Pump-probe microscopy non-destructively differentiates eumelanin and pheomelanin and can be used to quantify melanin distributions in thin biopsy slices. Here we have extended that work for imaging eumelanin and pheomelanin distributions on a sub-cellular scale, allowing elucidation of characteristi...

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Main Authors:	Simpson, Mary Jane, Wilson, Jesse W., Phipps, M. Anthony, Robles, Francisco E., Selim, M. Angelica, Warren, Warren S.
Format:	Online
Language:	English
Published:	2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3652914/

id	pubmed-3652914
recordtype	oai_dc
spelling	pubmed-36529142014-01-01 Nonlinear microscopy of eumelanin and pheomelanin with sub-cellular resolution Simpson, Mary Jane Wilson, Jesse W. Phipps, M. Anthony Robles, Francisco E. Selim, M. Angelica Warren, Warren S. Article Pump-probe microscopy non-destructively differentiates eumelanin and pheomelanin and can be used to quantify melanin distributions in thin biopsy slices. Here we have extended that work for imaging eumelanin and pheomelanin distributions on a sub-cellular scale, allowing elucidation of characteristics of different cell types. The results show that melanin heterogeneity, previously found to be characteristic of melanomas, persists on the sub-cellular scale. We have also found spectral changes associated with melanin located in melanophages, which could potentially differentiate invasive pigmented melanocytes from melanophages without immunohistochemical staining. 2013-01-25 2013-07 /pmc/articles/PMC3652914/ /pubmed/23353985 http://dx.doi.org/10.1038/jid.2013.37 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Simpson, Mary Jane Wilson, Jesse W. Phipps, M. Anthony Robles, Francisco E. Selim, M. Angelica Warren, Warren S.
spellingShingle	Simpson, Mary Jane Wilson, Jesse W. Phipps, M. Anthony Robles, Francisco E. Selim, M. Angelica Warren, Warren S. Nonlinear microscopy of eumelanin and pheomelanin with sub-cellular resolution
author_facet	Simpson, Mary Jane Wilson, Jesse W. Phipps, M. Anthony Robles, Francisco E. Selim, M. Angelica Warren, Warren S.
author_sort	Simpson, Mary Jane
title	Nonlinear microscopy of eumelanin and pheomelanin with sub-cellular resolution
title_short	Nonlinear microscopy of eumelanin and pheomelanin with sub-cellular resolution
title_full	Nonlinear microscopy of eumelanin and pheomelanin with sub-cellular resolution
title_fullStr	Nonlinear microscopy of eumelanin and pheomelanin with sub-cellular resolution
title_full_unstemmed	Nonlinear microscopy of eumelanin and pheomelanin with sub-cellular resolution
title_sort	nonlinear microscopy of eumelanin and pheomelanin with sub-cellular resolution
description	Pump-probe microscopy non-destructively differentiates eumelanin and pheomelanin and can be used to quantify melanin distributions in thin biopsy slices. Here we have extended that work for imaging eumelanin and pheomelanin distributions on a sub-cellular scale, allowing elucidation of characteristics of different cell types. The results show that melanin heterogeneity, previously found to be characteristic of melanomas, persists on the sub-cellular scale. We have also found spectral changes associated with melanin located in melanophages, which could potentially differentiate invasive pigmented melanocytes from melanophages without immunohistochemical staining.
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3652914/
_version_	1611977450880761856

Nonlinear microscopy of eumelanin and pheomelanin with sub-cellular resolution

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