Molecular Cloning and Immunochemical Characterization of a New Japanese Cedar Pollen Allergen Homologous to Plant Subtilisin-Like Serine Protease

Protease activities in allergen sources are thought to be involved in triggering allergic inflammation through the disruption of epithelial barrier or the induction of proinflammatory cytokines. Protease allergens may also work as type 2 helper T cell (TH2) adjuvants through the cleavage of cell sur...

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Main Authors:	Nour Ibrahim, Ahmed Ragaa, Kawamoto, Seiji, Mizuno, Keisuke, Shimada, Yayoi, Rikimaru, Satoshi, Onishi, Nobukazu, Hashimoto, Kunihiko, Aki, Tsunehiro, Hayashi, Takaharu, Ono, Kazuhisa
Format:	Online
Language:	English
Published:	World Allergy Organization 2010
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651113/

id	pubmed-3651113
recordtype	oai_dc
spelling	pubmed-36511132013-07-12 Molecular Cloning and Immunochemical Characterization of a New Japanese Cedar Pollen Allergen Homologous to Plant Subtilisin-Like Serine Protease Nour Ibrahim, Ahmed Ragaa Kawamoto, Seiji Mizuno, Keisuke Shimada, Yayoi Rikimaru, Satoshi Onishi, Nobukazu Hashimoto, Kunihiko Aki, Tsunehiro Hayashi, Takaharu Ono, Kazuhisa Original Research Protease activities in allergen sources are thought to be involved in triggering allergic inflammation through the disruption of epithelial barrier or the induction of proinflammatory cytokines. Protease allergens may also work as type 2 helper T cell (TH2) adjuvants through the cleavage of cell surface receptors. Here, we report molecular cloning and immunochemical characterization of a new Japanese cedar (Cryptomeria japonica) pollen allergen (CPA9) homologous to serine protease, which is initially found as a high IgE-binding spot on our two-dimensional (2-D) IgE immunoblotting map. The cpa9 cDNA encoded a 757 amino acid polypeptide showing a significant sequence identity with plant subtilisin-like serine protease family members including melon major allergen Cuc m 1. We found that native CPA9 purified from C. japonica pollen showed a high IgE-binding frequency and IgE cross-reactivity with melon extract. World Allergy Organization 2010-11-15 /pmc/articles/PMC3651113/ /pubmed/23282945 http://dx.doi.org/10.1097/WOX.0b013e318201d81d Text en Copyright ©2010 World Allergy Organization; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Nour Ibrahim, Ahmed Ragaa Kawamoto, Seiji Mizuno, Keisuke Shimada, Yayoi Rikimaru, Satoshi Onishi, Nobukazu Hashimoto, Kunihiko Aki, Tsunehiro Hayashi, Takaharu Ono, Kazuhisa
spellingShingle	Nour Ibrahim, Ahmed Ragaa Kawamoto, Seiji Mizuno, Keisuke Shimada, Yayoi Rikimaru, Satoshi Onishi, Nobukazu Hashimoto, Kunihiko Aki, Tsunehiro Hayashi, Takaharu Ono, Kazuhisa Molecular Cloning and Immunochemical Characterization of a New Japanese Cedar Pollen Allergen Homologous to Plant Subtilisin-Like Serine Protease
author_facet	Nour Ibrahim, Ahmed Ragaa Kawamoto, Seiji Mizuno, Keisuke Shimada, Yayoi Rikimaru, Satoshi Onishi, Nobukazu Hashimoto, Kunihiko Aki, Tsunehiro Hayashi, Takaharu Ono, Kazuhisa
author_sort	Nour Ibrahim, Ahmed Ragaa
title	Molecular Cloning and Immunochemical Characterization of a New Japanese Cedar Pollen Allergen Homologous to Plant Subtilisin-Like Serine Protease
title_short	Molecular Cloning and Immunochemical Characterization of a New Japanese Cedar Pollen Allergen Homologous to Plant Subtilisin-Like Serine Protease
title_full	Molecular Cloning and Immunochemical Characterization of a New Japanese Cedar Pollen Allergen Homologous to Plant Subtilisin-Like Serine Protease
title_fullStr	Molecular Cloning and Immunochemical Characterization of a New Japanese Cedar Pollen Allergen Homologous to Plant Subtilisin-Like Serine Protease
title_full_unstemmed	Molecular Cloning and Immunochemical Characterization of a New Japanese Cedar Pollen Allergen Homologous to Plant Subtilisin-Like Serine Protease
title_sort	molecular cloning and immunochemical characterization of a new japanese cedar pollen allergen homologous to plant subtilisin-like serine protease
description	Protease activities in allergen sources are thought to be involved in triggering allergic inflammation through the disruption of epithelial barrier or the induction of proinflammatory cytokines. Protease allergens may also work as type 2 helper T cell (TH2) adjuvants through the cleavage of cell surface receptors. Here, we report molecular cloning and immunochemical characterization of a new Japanese cedar (Cryptomeria japonica) pollen allergen (CPA9) homologous to serine protease, which is initially found as a high IgE-binding spot on our two-dimensional (2-D) IgE immunoblotting map. The cpa9 cDNA encoded a 757 amino acid polypeptide showing a significant sequence identity with plant subtilisin-like serine protease family members including melon major allergen Cuc m 1. We found that native CPA9 purified from C. japonica pollen showed a high IgE-binding frequency and IgE cross-reactivity with melon extract.
publisher	World Allergy Organization
publishDate	2010
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3651113/
_version_	1611976981053702144

Molecular Cloning and Immunochemical Characterization of a New Japanese Cedar Pollen Allergen Homologous to Plant Subtilisin-Like Serine Protease

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