A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves

A one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the co...

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Main Authors:	Frascella, Francesca, Ricciardi, Serena, Rivolo, Paola, Moi, Valeria, Giorgis, Fabrizio, Descrovi, Emiliano, Michelotti, Francesco, Munzert, Peter, Danz, Norbert, Napione, Lucia, Alvaro, Maria, Bussolino, Federico
Format:	Online
Language:	English
Published:	Molecular Diversity Preservation International (MDPI) 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3649429/

id	pubmed-3649429
recordtype	oai_dc
spelling	pubmed-36494292013-06-04 A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves Frascella, Francesca Ricciardi, Serena Rivolo, Paola Moi, Valeria Giorgis, Fabrizio Descrovi, Emiliano Michelotti, Francesco Munzert, Peter Danz, Norbert Napione, Lucia Alvaro, Maria Bussolino, Federico Article A one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the covalent binding of orienting proteins needed for a subsequent grafting of antibodies in an immunoassay detection scheme. The polymeric layer is impregnated with Cy3 dye, in such a way that the photonic structure can exhibit an emissive behavior. The BSW-coupled fluorescence shift is used as a means for detecting refractive index variations occurring at the 1DPC surface, according to a label-free concept. The proposed working principle is successfully demonstrated in real-time tracking of protein G covalent binding on the 1DPC surface within a fluidic cell. Molecular Diversity Preservation International (MDPI) 2013-02-05 /pmc/articles/PMC3649429/ /pubmed/23385414 http://dx.doi.org/10.3390/s130202011 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Frascella, Francesca Ricciardi, Serena Rivolo, Paola Moi, Valeria Giorgis, Fabrizio Descrovi, Emiliano Michelotti, Francesco Munzert, Peter Danz, Norbert Napione, Lucia Alvaro, Maria Bussolino, Federico
spellingShingle	Frascella, Francesca Ricciardi, Serena Rivolo, Paola Moi, Valeria Giorgis, Fabrizio Descrovi, Emiliano Michelotti, Francesco Munzert, Peter Danz, Norbert Napione, Lucia Alvaro, Maria Bussolino, Federico A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves
author_facet	Frascella, Francesca Ricciardi, Serena Rivolo, Paola Moi, Valeria Giorgis, Fabrizio Descrovi, Emiliano Michelotti, Francesco Munzert, Peter Danz, Norbert Napione, Lucia Alvaro, Maria Bussolino, Federico
author_sort	Frascella, Francesca
title	A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves
title_short	A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves
title_full	A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves
title_fullStr	A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves
title_full_unstemmed	A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves
title_sort	fluorescent one-dimensional photonic crystal for label-free biosensing based on bloch surface waves
description	A one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the covalent binding of orienting proteins needed for a subsequent grafting of antibodies in an immunoassay detection scheme. The polymeric layer is impregnated with Cy3 dye, in such a way that the photonic structure can exhibit an emissive behavior. The BSW-coupled fluorescence shift is used as a means for detecting refractive index variations occurring at the 1DPC surface, according to a label-free concept. The proposed working principle is successfully demonstrated in real-time tracking of protein G covalent binding on the 1DPC surface within a fluidic cell.
publisher	Molecular Diversity Preservation International (MDPI)
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3649429/
_version_	1611976505132318720

A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves

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