Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

Muscle regeneration in the adult is performed by resident stem cells called satellite cells. Satellite cells are defined by their position between the basal lamina and the sarcolemma of each myofiber. Current knowledge of their behavior heavily relies on the use of the single myofiber isolation prot...

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Bibliographic Details
Main Authors: Pasut, Alessandra, Jones, Andrew E., Rudnicki, Michael A.
Format: Online
Language:English
Published: MyJove Corporation 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3639710/