Phosphoinositide 3-Kinase Dependent Inhibition as a Broad Basis for Opponent Coding in Mammalian Olfactory Receptor Neurons

Phosphoinositide 3-kinase (PI3K) signaling has been implicated in mediating inhibitory odorant input to mammalian olfactory receptor neurons (ORNs). To better understand the breadth of such inhibition in odor coding, we screened a panel of odorants representing different chemical classes, as well as...

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Main Authors:	Ukhanov, Kirill, Corey, Elizabeth A., Ache, Barry W.
Format:	Online
Language:	English
Published:	Public Library of Science 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621990/

id	pubmed-3621990
recordtype	oai_dc
spelling	pubmed-36219902013-04-12 Phosphoinositide 3-Kinase Dependent Inhibition as a Broad Basis for Opponent Coding in Mammalian Olfactory Receptor Neurons Ukhanov, Kirill Corey, Elizabeth A. Ache, Barry W. Research Article Phosphoinositide 3-kinase (PI3K) signaling has been implicated in mediating inhibitory odorant input to mammalian olfactory receptor neurons (ORNs). To better understand the breadth of such inhibition in odor coding, we screened a panel of odorants representing different chemical classes, as well as odorants known to occur in a natural odor object (tomato), for their ability to rapidly activate PI3K-dependent inhibitory signaling. Odorants were screened on dissociated native rat ORNs before and after pre-incubation with the PI3K-isoform specific blockers AS252424 and TGX221. Many different odorants increased their excitatory strength for particular ORNs following PI3K blockade in a manner consistent with activating PI3K-dependent inhibitory signaling in those cells. The PI3K-dependent inhibitory odorants overlapped with conventional excitatory odorants, but did not share the same bias, indicating partial partitioning of the odor space. Finding that PI3K-dependent inhibition can be activated by a wide range of otherwise conventional excitatory odorants strongly implies PI3K-dependent inhibition provides a broad basis for opponent coding in mammalian ORNs. Public Library of Science 2013-04-09 /pmc/articles/PMC3621990/ /pubmed/23585911 http://dx.doi.org/10.1371/journal.pone.0061553 Text en © 2013 Ukhanov et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Ukhanov, Kirill Corey, Elizabeth A. Ache, Barry W.
spellingShingle	Ukhanov, Kirill Corey, Elizabeth A. Ache, Barry W. Phosphoinositide 3-Kinase Dependent Inhibition as a Broad Basis for Opponent Coding in Mammalian Olfactory Receptor Neurons
author_facet	Ukhanov, Kirill Corey, Elizabeth A. Ache, Barry W.
author_sort	Ukhanov, Kirill
title	Phosphoinositide 3-Kinase Dependent Inhibition as a Broad Basis for Opponent Coding in Mammalian Olfactory Receptor Neurons
title_short	Phosphoinositide 3-Kinase Dependent Inhibition as a Broad Basis for Opponent Coding in Mammalian Olfactory Receptor Neurons
title_full	Phosphoinositide 3-Kinase Dependent Inhibition as a Broad Basis for Opponent Coding in Mammalian Olfactory Receptor Neurons
title_fullStr	Phosphoinositide 3-Kinase Dependent Inhibition as a Broad Basis for Opponent Coding in Mammalian Olfactory Receptor Neurons
title_full_unstemmed	Phosphoinositide 3-Kinase Dependent Inhibition as a Broad Basis for Opponent Coding in Mammalian Olfactory Receptor Neurons
title_sort	phosphoinositide 3-kinase dependent inhibition as a broad basis for opponent coding in mammalian olfactory receptor neurons
description	Phosphoinositide 3-kinase (PI3K) signaling has been implicated in mediating inhibitory odorant input to mammalian olfactory receptor neurons (ORNs). To better understand the breadth of such inhibition in odor coding, we screened a panel of odorants representing different chemical classes, as well as odorants known to occur in a natural odor object (tomato), for their ability to rapidly activate PI3K-dependent inhibitory signaling. Odorants were screened on dissociated native rat ORNs before and after pre-incubation with the PI3K-isoform specific blockers AS252424 and TGX221. Many different odorants increased their excitatory strength for particular ORNs following PI3K blockade in a manner consistent with activating PI3K-dependent inhibitory signaling in those cells. The PI3K-dependent inhibitory odorants overlapped with conventional excitatory odorants, but did not share the same bias, indicating partial partitioning of the odor space. Finding that PI3K-dependent inhibition can be activated by a wide range of otherwise conventional excitatory odorants strongly implies PI3K-dependent inhibition provides a broad basis for opponent coding in mammalian ORNs.
publisher	Public Library of Science
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3621990/
_version_	1611969142879944704

Phosphoinositide 3-Kinase Dependent Inhibition as a Broad Basis for Opponent Coding in Mammalian Olfactory Receptor Neurons

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