Protein profile of rice (Oryza sativa) seeds
Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecu...
| Main Authors: | , , , , , |
|---|---|
| Format: | Online |
| Language: | English |
| Published: |
Sociedade Brasileira de Genética
2013
|
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615530/ |
| id |
pubmed-3615530 |
|---|---|
| recordtype |
oai_dc |
| spelling |
pubmed-36155302013-04-08 Protein profile of rice (Oryza sativa) seeds Yang, Yanhua Dai, Li Xia, Hengchuan Zhu, Keming Liu, Haijun Chen, Keping Plant Genetics Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4–7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic and were related to binding, catalytic, cellular or metabolic processes. These results expand our knowledge of the rice proteome and improve our understanding of the cellular biology of rice seeds. Sociedade Brasileira de Genética 2013-03-04 2013-03 /pmc/articles/PMC3615530/ /pubmed/23569412 http://dx.doi.org/10.1590/S1415-47572013000100012 Text en Copyright © 2013, Sociedade Brasileira de Genética. License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Yang, Yanhua Dai, Li Xia, Hengchuan Zhu, Keming Liu, Haijun Chen, Keping |
| spellingShingle |
Yang, Yanhua Dai, Li Xia, Hengchuan Zhu, Keming Liu, Haijun Chen, Keping Protein profile of rice (Oryza sativa) seeds |
| author_facet |
Yang, Yanhua Dai, Li Xia, Hengchuan Zhu, Keming Liu, Haijun Chen, Keping |
| author_sort |
Yang, Yanhua |
| title |
Protein profile of rice (Oryza sativa) seeds |
| title_short |
Protein profile of rice (Oryza sativa) seeds |
| title_full |
Protein profile of rice (Oryza sativa) seeds |
| title_fullStr |
Protein profile of rice (Oryza sativa) seeds |
| title_full_unstemmed |
Protein profile of rice (Oryza sativa) seeds |
| title_sort |
protein profile of rice (oryza sativa) seeds |
| description |
Seeds are the most important plant storage organ and play a central role in the life cycle of plants. Since little is known about the protein composition of rice (Oryza sativa) seeds, in this work we used proteomic methods to obtain a reference map of rice seed proteins and identify important molecules. Overall, 480 reproducible protein spots were detected by two-dimensional electrophoresis on pH 4–7 gels and 302 proteins were identified by MALDI-TOF MS and database searches. Together, these proteins represented 252 gene products and were classified into 12 functional categories, most of which were involved in metabolic pathways. Database searches combined with hydropathy plots and gene ontology analysis showed that most rice seed proteins were hydrophilic and were related to binding, catalytic, cellular or metabolic processes. These results expand our knowledge of the rice proteome and improve our understanding of the cellular biology of rice seeds. |
| publisher |
Sociedade Brasileira de Genética |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615530/ |
| _version_ |
1611967291468021760 |