Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera
Extracts of Chaetomium globosum EF18, isolated as endophytic fungus from Withania somnifera, were found effective against Sclerotinia sclerotiorum. Ethyl acetate and methanol extracts were more effective than hexane extract showing >80% growth inhibition. Bioactive compound (antibiotic Sch 210971...
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| Format: | Online |
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Springer International Publishing AG
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601270/ |
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pubmed-36012702013-03-19 Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera Kumar, Susheel Kaushik, Nutan Proksch, Peter Research Extracts of Chaetomium globosum EF18, isolated as endophytic fungus from Withania somnifera, were found effective against Sclerotinia sclerotiorum. Ethyl acetate and methanol extracts were more effective than hexane extract showing >80% growth inhibition. Bioactive compound (antibiotic Sch 210971, m/z 445 and λmax 290) having antifungal activity against S. sclerotiorum has been isolated in pure form from the ethyl acetate extract following bioassay guided fractionation. Apart from this compound other fractions of polar to medium polarity were also found effective. Fraction no. VIII from VLC (Vacuum liquid chromatography) column of ethyl acetate extract was most active having IC50 value 35.4 μg/ml. Springer International Publishing AG 2013-02-06 /pmc/articles/PMC3601270/ /pubmed/23519550 http://dx.doi.org/10.1186/2193-1801-2-37 Text en © Kumar et al; licensee Springer. 2013 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Kumar, Susheel Kaushik, Nutan Proksch, Peter |
| spellingShingle |
Kumar, Susheel Kaushik, Nutan Proksch, Peter Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera |
| author_facet |
Kumar, Susheel Kaushik, Nutan Proksch, Peter |
| author_sort |
Kumar, Susheel |
| title |
Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera |
| title_short |
Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera |
| title_full |
Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera |
| title_fullStr |
Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera |
| title_full_unstemmed |
Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum from Withania somnifera |
| title_sort |
identification of antifungal principle in the solvent extract of an endophytic fungus chaetomium globosum from withania somnifera |
| description |
Extracts of Chaetomium globosum EF18, isolated as endophytic fungus from Withania somnifera, were found effective against Sclerotinia sclerotiorum. Ethyl acetate and methanol extracts were more effective than hexane extract showing >80% growth inhibition. Bioactive compound (antibiotic Sch 210971, m/z 445 and λmax 290) having antifungal activity against S. sclerotiorum has been isolated in pure form from the ethyl acetate extract following bioassay guided fractionation. Apart from this compound other fractions of polar to medium polarity were also found effective. Fraction no. VIII from VLC (Vacuum liquid chromatography) column of ethyl acetate extract was most active having IC50 value 35.4 μg/ml. |
| publisher |
Springer International Publishing AG |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601270/ |
| _version_ |
1611963581528539136 |