Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal models of acute ischemic stroke allows to confirm successful arterial occlusion and exclude subarachnoid hemorrhage. Cerebral perfusion monitoring can...

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Main Authors:	Beretta, Simone, Riva, Matteo, Carone, Davide, Cuccione, Elisa, Padovano, Giada, Rodriguez Menendez, Virginia, Pappadá, Giovanni B., Versace, Alessandro, Giussani, Carlo, Sganzerla, Erik P., Ferrarese, Carlo
Format:	Online
Language:	English
Published:	MyJove Corporation 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601211/

id	pubmed-3601211
recordtype	oai_dc
spelling	pubmed-36012112015-02-17 Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion Beretta, Simone Riva, Matteo Carone, Davide Cuccione, Elisa Padovano, Giada Rodriguez Menendez, Virginia Pappadá, Giovanni B. Versace, Alessandro Giussani, Carlo Sganzerla, Erik P. Ferrarese, Carlo Medicine The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal models of acute ischemic stroke allows to confirm successful arterial occlusion and exclude subarachnoid hemorrhage. Cerebral perfusion monitoring can also be used to study intracranial collateral circulation, which is emerging as a powerful determinant of stroke outcome and a possible therapeutic target. Despite a recognized role of Laser Doppler perfusion monitoring as part of the current guidelines for experimental cerebral ischemia, a number of technical difficulties exist that limit its widespread use. One of the major issues is obtaining a secure and prolonged attachment of a deep-penetration Laser Doppler probe to the animal skull. In this video, we show our optimized system for cerebral perfusion monitoring during transient middle cerebral artery occlusion by intraluminal filament in the rat. We developed in-house a simple method to obtain a custom made holder for twin-fibre (deep-penetration) Laser Doppler probes, which allow multi-site monitoring if needed. A continuous and prolonged monitoring of cerebral perfusion could easily be obtained over the intact skull. MyJove Corporation 2013-02-17 /pmc/articles/PMC3601211/ /pubmed/23439069 http://dx.doi.org/10.3791/50214 Text en Copyright © 2013, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Beretta, Simone Riva, Matteo Carone, Davide Cuccione, Elisa Padovano, Giada Rodriguez Menendez, Virginia Pappadá, Giovanni B. Versace, Alessandro Giussani, Carlo Sganzerla, Erik P. Ferrarese, Carlo
spellingShingle	Beretta, Simone Riva, Matteo Carone, Davide Cuccione, Elisa Padovano, Giada Rodriguez Menendez, Virginia Pappadá, Giovanni B. Versace, Alessandro Giussani, Carlo Sganzerla, Erik P. Ferrarese, Carlo Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion
author_facet	Beretta, Simone Riva, Matteo Carone, Davide Cuccione, Elisa Padovano, Giada Rodriguez Menendez, Virginia Pappadá, Giovanni B. Versace, Alessandro Giussani, Carlo Sganzerla, Erik P. Ferrarese, Carlo
author_sort	Beretta, Simone
title	Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion
title_short	Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion
title_full	Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion
title_fullStr	Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion
title_full_unstemmed	Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion
title_sort	optimized system for cerebral perfusion monitoring in the rat stroke model of intraluminal middle cerebral artery occlusion
description	The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal models of acute ischemic stroke allows to confirm successful arterial occlusion and exclude subarachnoid hemorrhage. Cerebral perfusion monitoring can also be used to study intracranial collateral circulation, which is emerging as a powerful determinant of stroke outcome and a possible therapeutic target. Despite a recognized role of Laser Doppler perfusion monitoring as part of the current guidelines for experimental cerebral ischemia, a number of technical difficulties exist that limit its widespread use. One of the major issues is obtaining a secure and prolonged attachment of a deep-penetration Laser Doppler probe to the animal skull. In this video, we show our optimized system for cerebral perfusion monitoring during transient middle cerebral artery occlusion by intraluminal filament in the rat. We developed in-house a simple method to obtain a custom made holder for twin-fibre (deep-penetration) Laser Doppler probes, which allow multi-site monitoring if needed. A continuous and prolonged monitoring of cerebral perfusion could easily be obtained over the intact skull.
publisher	MyJove Corporation
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3601211/
_version_	1611963553095352320

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

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