UV damage regulates alternative polyadenylation of the RPB2 gene in yeast

Alternative polyadenylation (APA) is conserved in all eukaryotic cells. Selective use of polyadenylation sites appears to be a highly regulated process and contributes to human pathogenesis. In this article we report that the yeast RPB2 gene is alternatively polyadenylated, producing two mRNAs with...

Full description

Bibliographic Details
Main Authors:	Yu, Lijian, Volkert, Michael R.
Format:	Online
Language:	English
Published:	Oxford University Press 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597686/

id	pubmed-3597686
recordtype	oai_dc
spelling	pubmed-35976862013-03-15 UV damage regulates alternative polyadenylation of the RPB2 gene in yeast Yu, Lijian Volkert, Michael R. Genome Integrity, Repair and Replication Alternative polyadenylation (APA) is conserved in all eukaryotic cells. Selective use of polyadenylation sites appears to be a highly regulated process and contributes to human pathogenesis. In this article we report that the yeast RPB2 gene is alternatively polyadenylated, producing two mRNAs with different lengths of 3′UTR. In normally growing wild-type cells, polyadenylation preferentially uses the promoter-proximal poly(A) site. After UV damage transcription of RPB2 is initially inhibited. As transcription recovers, the promoter-distal poly(A) site is preferentially used instead, producing more of a longer form of RPB2 mRNA. We show that the relative increase in the long RPB2 mRNA is not caused by increased mRNA stability, supporting the preferential usage of the distal poly(A) site during transcription recovery. We demonstrate that the 3′UTR of RPB2 is sufficient for this UV-induced regulation of APA. We present evidence that while transcription initiation rates do not seem to influence selection of the poly(A) sites of RPB2, the rate of transcription elongation is an important determinant. Oxford University Press 2013-03 2013-01-25 /pmc/articles/PMC3597686/ /pubmed/23355614 http://dx.doi.org/10.1093/nar/gkt020 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Yu, Lijian Volkert, Michael R.
spellingShingle	Yu, Lijian Volkert, Michael R. UV damage regulates alternative polyadenylation of the RPB2 gene in yeast
author_facet	Yu, Lijian Volkert, Michael R.
author_sort	Yu, Lijian
title	UV damage regulates alternative polyadenylation of the RPB2 gene in yeast
title_short	UV damage regulates alternative polyadenylation of the RPB2 gene in yeast
title_full	UV damage regulates alternative polyadenylation of the RPB2 gene in yeast
title_fullStr	UV damage regulates alternative polyadenylation of the RPB2 gene in yeast
title_full_unstemmed	UV damage regulates alternative polyadenylation of the RPB2 gene in yeast
title_sort	uv damage regulates alternative polyadenylation of the rpb2 gene in yeast
description	Alternative polyadenylation (APA) is conserved in all eukaryotic cells. Selective use of polyadenylation sites appears to be a highly regulated process and contributes to human pathogenesis. In this article we report that the yeast RPB2 gene is alternatively polyadenylated, producing two mRNAs with different lengths of 3′UTR. In normally growing wild-type cells, polyadenylation preferentially uses the promoter-proximal poly(A) site. After UV damage transcription of RPB2 is initially inhibited. As transcription recovers, the promoter-distal poly(A) site is preferentially used instead, producing more of a longer form of RPB2 mRNA. We show that the relative increase in the long RPB2 mRNA is not caused by increased mRNA stability, supporting the preferential usage of the distal poly(A) site during transcription recovery. We demonstrate that the 3′UTR of RPB2 is sufficient for this UV-induced regulation of APA. We present evidence that while transcription initiation rates do not seem to influence selection of the poly(A) sites of RPB2, the rate of transcription elongation is an important determinant.
publisher	Oxford University Press
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3597686/
_version_	1611962177582792704

UV damage regulates alternative polyadenylation of the RPB2 gene in yeast

Similar Items