All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro
All-trans retinoid acid (ATRA) is one of the most potent and most thoroughly studied differentiation inducers that induce the differentiation and apoptosis of glioma cells. However, the effect of ATRA on angiogenesis of glioma remains poorly understood. We examined the effect of ATRA on the expressi...
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Editorial Department of Journal of Biomedical Research
2013
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| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596755/ |
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pubmed-35967552013-04-02 All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro Liang, Chen Guo, Shiwen Yang, Ling Research Paper All-trans retinoid acid (ATRA) is one of the most potent and most thoroughly studied differentiation inducers that induce the differentiation and apoptosis of glioma cells. However, the effect of ATRA on angiogenesis of glioma remains poorly understood. We examined the effect of ATRA on the expression of vascular endothelial growth factor (VEGF) in different glioma cell lines and investigated the underlying mechanism, intending to partially reveal the effects of ATRA on angiogenesis of glioma. Glioma cells were treated by ATRA at 5 and 10 µmol/L. The VEGF mRNA transcript levels were determined by real-time RT-PCR and the protein levels of VEGF in glioma cells were evaluated by Western blotting assays. Moreover, hypoxia-inducible factor-1α (HIF-1α) mRNA expression was analyzed by using real-time RT-PCR. After treatment with 5 and 10 µmol/L ATRA, the VEGF mRNA transcript levels in glioma cells increased remarkably, compared with that in the control group, and the relative protein expression of VEGF was also up-regulated. Meanwhile, the HIF-1α mRNA expression also increased. ATRA increases the expression of VEGF in glioma cells at both transcriptional and translational levels. Editorial Department of Journal of Biomedical Research 2013-01 2012-12-19 /pmc/articles/PMC3596755/ /pubmed/23554794 http://dx.doi.org/10.7555/JBR.27.20120048 Text en © 2013 by the Journal of Biomedical Research. All rights reserved. This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/ |
| repository_type |
Open Access Journal |
| institution_category |
Foreign Institution |
| institution |
US National Center for Biotechnology Information |
| building |
NCBI PubMed |
| collection |
Online Access |
| language |
English |
| format |
Online |
| author |
Liang, Chen Guo, Shiwen Yang, Ling |
| spellingShingle |
Liang, Chen Guo, Shiwen Yang, Ling All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro |
| author_facet |
Liang, Chen Guo, Shiwen Yang, Ling |
| author_sort |
Liang, Chen |
| title |
All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro |
| title_short |
All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro |
| title_full |
All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro |
| title_fullStr |
All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro |
| title_full_unstemmed |
All-trans retinoic acid upregulates VEGF expression in glioma cells in vitro |
| title_sort |
all-trans retinoic acid upregulates vegf expression in glioma cells in vitro |
| description |
All-trans retinoid acid (ATRA) is one of the most potent and most thoroughly studied differentiation inducers that induce the differentiation and apoptosis of glioma cells. However, the effect of ATRA on angiogenesis of glioma remains poorly understood. We examined the effect of ATRA on the expression of vascular endothelial growth factor (VEGF) in different glioma cell lines and investigated the underlying mechanism, intending to partially reveal the effects of ATRA on angiogenesis of glioma. Glioma cells were treated by ATRA at 5 and 10 µmol/L. The VEGF mRNA transcript levels were determined by real-time RT-PCR and the protein levels of VEGF in glioma cells were evaluated by Western blotting assays. Moreover, hypoxia-inducible factor-1α (HIF-1α) mRNA expression was analyzed by using real-time RT-PCR. After treatment with 5 and 10 µmol/L ATRA, the VEGF mRNA transcript levels in glioma cells increased remarkably, compared with that in the control group, and the relative protein expression of VEGF was also up-regulated. Meanwhile, the HIF-1α mRNA expression also increased. ATRA increases the expression of VEGF in glioma cells at both transcriptional and translational levels. |
| publisher |
Editorial Department of Journal of Biomedical Research |
| publishDate |
2013 |
| url |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596755/ |
| _version_ |
1611961890438643712 |