The role of microRNAs during the genesis of medulloblastomas induced by the hedgehog pathway

Constitutive hedgehog (Hh) signaling is associated with the genesis of medulloblastomas (MB). The objective of this study is to identify special microRNAs (miRNAs) regulated by the Hh pathway, and to clarify the role of miRNAs during the genesis of MB induced by sustained Hh activation. In the prima...

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Main Authors:	Luo, Xiaoju, Liu, Jun, Cheng, Steven Y
Format:	Online
Language:	English
Published:	Editorial Department of Journal of Biomedical Research 2011
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596675/

id	pubmed-3596675
recordtype	oai_dc
spelling	pubmed-35966752013-04-02 The role of microRNAs during the genesis of medulloblastomas induced by the hedgehog pathway Luo, Xiaoju Liu, Jun Cheng, Steven Y Research Paper Constitutive hedgehog (Hh) signaling is associated with the genesis of medulloblastomas (MB). The objective of this study is to identify special microRNAs (miRNAs) regulated by the Hh pathway, and to clarify the role of miRNAs during the genesis of MB induced by sustained Hh activation. In the primary screening, we used stem-loop RT-PCR to test the expression of 90 different miRNAs in the wildtype (WT) and Ptc-/- MEF cell lines. In the secondary screening, the miRNAs screened from the first screening were validated in the Sufu-/- MEF cell lines. We then verified the expression of miRNAs both in the normal cerebellar tissues and the MB induced by activated Hh pathway, and examined the expression of the other 21 miRNA members of the miR-154 cluster in the MB and normal cerebellum. In the first screening, 13 miRNAs showed significant differential expression in WT and Ptc-/- MEF cell lines, while 10 of them had significant difference in the Sufu-/- MEF cell line. Compared to the normal mouse cerebellum, only 2 miRNAs in 15 miRNAs were differentially expressed between the MB and normal cerebellar tissues. Among 21 members of the miR-154 cluster, 6 miRNAs were downregulated in the MB. Our study demonstrated that miR-154 may be regulated by the Hh pathway, and the activation of the Hh pathway led to the downregulation of the miR-154 cluster, resulting in the genesis of MB. Editorial Department of Journal of Biomedical Research 2011-01 /pmc/articles/PMC3596675/ /pubmed/23554670 http://dx.doi.org/10.1016/S1674-8301(11)60005-5 Text en © 2011 by the Journal of Biomedical Research. All rights reserved. This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Luo, Xiaoju Liu, Jun Cheng, Steven Y
spellingShingle	Luo, Xiaoju Liu, Jun Cheng, Steven Y The role of microRNAs during the genesis of medulloblastomas induced by the hedgehog pathway
author_facet	Luo, Xiaoju Liu, Jun Cheng, Steven Y
author_sort	Luo, Xiaoju
title	The role of microRNAs during the genesis of medulloblastomas induced by the hedgehog pathway
title_short	The role of microRNAs during the genesis of medulloblastomas induced by the hedgehog pathway
title_full	The role of microRNAs during the genesis of medulloblastomas induced by the hedgehog pathway
title_fullStr	The role of microRNAs during the genesis of medulloblastomas induced by the hedgehog pathway
title_full_unstemmed	The role of microRNAs during the genesis of medulloblastomas induced by the hedgehog pathway
title_sort	role of micrornas during the genesis of medulloblastomas induced by the hedgehog pathway
description	Constitutive hedgehog (Hh) signaling is associated with the genesis of medulloblastomas (MB). The objective of this study is to identify special microRNAs (miRNAs) regulated by the Hh pathway, and to clarify the role of miRNAs during the genesis of MB induced by sustained Hh activation. In the primary screening, we used stem-loop RT-PCR to test the expression of 90 different miRNAs in the wildtype (WT) and Ptc-/- MEF cell lines. In the secondary screening, the miRNAs screened from the first screening were validated in the Sufu-/- MEF cell lines. We then verified the expression of miRNAs both in the normal cerebellar tissues and the MB induced by activated Hh pathway, and examined the expression of the other 21 miRNA members of the miR-154 cluster in the MB and normal cerebellum. In the first screening, 13 miRNAs showed significant differential expression in WT and Ptc-/- MEF cell lines, while 10 of them had significant difference in the Sufu-/- MEF cell line. Compared to the normal mouse cerebellum, only 2 miRNAs in 15 miRNAs were differentially expressed between the MB and normal cerebellar tissues. Among 21 members of the miR-154 cluster, 6 miRNAs were downregulated in the MB. Our study demonstrated that miR-154 may be regulated by the Hh pathway, and the activation of the Hh pathway led to the downregulation of the miR-154 cluster, resulting in the genesis of MB.
publisher	Editorial Department of Journal of Biomedical Research
publishDate	2011
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3596675/
_version_	1611961847225778176

The role of microRNAs during the genesis of medulloblastomas induced by the hedgehog pathway

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