Sequence verification of synthetic DNA by assembly of sequencing reads

Gene synthesis attempts to assemble user-defined DNA sequences with base-level precision. Verifying the sequences of construction intermediates and the final product of a gene synthesis project is a critical part of the workflow, yet one that has received the least attention. Sequence validation is...

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Bibliographic Details
Main Authors: Wilson, Mandy L., Cai, Yizhi, Hanlon, Regina, Taylor, Samantha, Chevreux, Bastien, Setubal, João C., Tyler, Brett M., Peccoud, Jean
Format: Online
Language:English
Published: Oxford University Press 2013
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592409/