Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A

Streptococcus mutans produces 3 types of glucosyltransferases (GTFs), whose cooperative action is essential for cellular adhesion. The recombinase A (RecA) protein is required for homologous recombination. In our previous study, we isolated several strains with a smooth colony morphology and low GT...

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Main Authors:	Inagaki, Satoko, Fujita, Kazuyo, Takashima, Yukiko, Nagayama, Kayoko, Ardin, Arifah C., Matsumi, Yuki, Matsumoto-Nakano, Michiyo
Format:	Online
Language:	English
Published:	Hindawi Publishing Corporation 2013
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586492/

id	pubmed-3586492
recordtype	oai_dc
spelling	pubmed-35864922013-03-09 Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A Inagaki, Satoko Fujita, Kazuyo Takashima, Yukiko Nagayama, Kayoko Ardin, Arifah C. Matsumi, Yuki Matsumoto-Nakano, Michiyo Research Article Streptococcus mutans produces 3 types of glucosyltransferases (GTFs), whose cooperative action is essential for cellular adhesion. The recombinase A (RecA) protein is required for homologous recombination. In our previous study, we isolated several strains with a smooth colony morphology and low GTF activity, characteristics speculated to be derived from the GTF fusions. The purpose of the present study was to investigate the mechanism of those fusions. S. mutans strain MT8148 was grown in the presence of recombinant RecA (rRecA) protein, after which smooth colonies were isolated. The biological functions and sequences of the gtfB and gtfC genes of this as well as other clinical strains were determined. The sucrose-dependent adherence rates of those strains were reduced as compared to that of MT8148. Determination of the sequences of the gtfB and gtfC genes showed that an approximately 3500 bp region was deleted from the area between them. Furthermore, expression of the recA gene was elevated in those strains as compared to MT8148. These results suggest that RecA has an important role in fusions of gtfB and gtfC genes, leading to alteration of colony morphology and reduction in sucrose-dependent adhesion. Hindawi Publishing Corporation 2013-02-17 /pmc/articles/PMC3586492/ /pubmed/23476132 http://dx.doi.org/10.1155/2013/405075 Text en Copyright © 2013 Satoko Inagaki et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	Inagaki, Satoko Fujita, Kazuyo Takashima, Yukiko Nagayama, Kayoko Ardin, Arifah C. Matsumi, Yuki Matsumoto-Nakano, Michiyo
spellingShingle	Inagaki, Satoko Fujita, Kazuyo Takashima, Yukiko Nagayama, Kayoko Ardin, Arifah C. Matsumi, Yuki Matsumoto-Nakano, Michiyo Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A
author_facet	Inagaki, Satoko Fujita, Kazuyo Takashima, Yukiko Nagayama, Kayoko Ardin, Arifah C. Matsumi, Yuki Matsumoto-Nakano, Michiyo
author_sort	Inagaki, Satoko
title	Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A
title_short	Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A
title_full	Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A
title_fullStr	Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A
title_full_unstemmed	Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A
title_sort	regulation of recombination between gtfb/gtfc genes in streptococcus mutans by recombinase a
description	Streptococcus mutans produces 3 types of glucosyltransferases (GTFs), whose cooperative action is essential for cellular adhesion. The recombinase A (RecA) protein is required for homologous recombination. In our previous study, we isolated several strains with a smooth colony morphology and low GTF activity, characteristics speculated to be derived from the GTF fusions. The purpose of the present study was to investigate the mechanism of those fusions. S. mutans strain MT8148 was grown in the presence of recombinant RecA (rRecA) protein, after which smooth colonies were isolated. The biological functions and sequences of the gtfB and gtfC genes of this as well as other clinical strains were determined. The sucrose-dependent adherence rates of those strains were reduced as compared to that of MT8148. Determination of the sequences of the gtfB and gtfC genes showed that an approximately 3500 bp region was deleted from the area between them. Furthermore, expression of the recA gene was elevated in those strains as compared to MT8148. These results suggest that RecA has an important role in fusions of gtfB and gtfC genes, leading to alteration of colony morphology and reduction in sucrose-dependent adhesion.
publisher	Hindawi Publishing Corporation
publishDate	2013
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586492/
_version_	1611958955178721280

Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A

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