The relationship between tissue oxygenation and redox status using magnetic resonance imaging

The recent development of a bi-modality magnetic resonance imaging/electron paramagnetic resonance imaging (MRI/EPRI) platform has enabled longitudinal monitoring of both tumor oxygenation and redox status in murine cancer models. The current study used this imaging platform to test the hypothesis t...

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Main Authors:	HYODO, FUMINORI, DAVIS, RYAN M., HYODO, EMI, MATSUMOTO, SHINGO, KRISHNA, MURALI C., MITCHELL, JAMES B.
Format:	Online
Language:	English
Published:	D.A. Spandidos 2012
Online Access:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583655/

id	pubmed-3583655
recordtype	oai_dc
spelling	pubmed-35836552013-03-04 The relationship between tissue oxygenation and redox status using magnetic resonance imaging HYODO, FUMINORI DAVIS, RYAN M. HYODO, EMI MATSUMOTO, SHINGO KRISHNA, MURALI C. MITCHELL, JAMES B. Articles The recent development of a bi-modality magnetic resonance imaging/electron paramagnetic resonance imaging (MRI/EPRI) platform has enabled longitudinal monitoring of both tumor oxygenation and redox status in murine cancer models. The current study used this imaging platform to test the hypothesis that a more reducing tumor microenvironment accompanies the development of tumor hypoxia. To test this, the redox status of the tumor was measured using Tempol as a redox-sensitive MRI contrast agent, and tumor hypoxia was measured with Oxo63, which is an oxygen-sensitive EPRI spin probe. Images were acquired every 1–2 days in mice bearing SCCVII tumors. The median pO2 decreased from 14 mmHg at 7 days after tumor implantation to 7 mmHg at 15 days after implantation. Additionally, the hypoxic fraction, defined as the percentage of the tumor that exhibited a pO2<10 mmHg, increased with tumor size (from 10% at 500 mm3 to 60% at 3,500 mm3). The rate of Tempol reduction increased as a function of tumor volume (0.4 min−1 at 500 mm3 to 1.7 min−1 at 3,500 mm3), suggesting that the tumor microenvironment became more reduced as the tumor grew. The results show that rapid Tempol reduction correlates with decreased tumor oxygenation, and that the Tempol decay rate constant may be a surrogate marker for tumor hypoxia. D.A. Spandidos 2012-09-24 /pmc/articles/PMC3583655/ /pubmed/23007796 http://dx.doi.org/10.3892/ijo.2012.1638 Text en Copyright © 2012, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
repository_type	Open Access Journal
institution_category	Foreign Institution
institution	US National Center for Biotechnology Information
building	NCBI PubMed
collection	Online Access
language	English
format	Online
author	HYODO, FUMINORI DAVIS, RYAN M. HYODO, EMI MATSUMOTO, SHINGO KRISHNA, MURALI C. MITCHELL, JAMES B.
spellingShingle	HYODO, FUMINORI DAVIS, RYAN M. HYODO, EMI MATSUMOTO, SHINGO KRISHNA, MURALI C. MITCHELL, JAMES B. The relationship between tissue oxygenation and redox status using magnetic resonance imaging
author_facet	HYODO, FUMINORI DAVIS, RYAN M. HYODO, EMI MATSUMOTO, SHINGO KRISHNA, MURALI C. MITCHELL, JAMES B.
author_sort	HYODO, FUMINORI
title	The relationship between tissue oxygenation and redox status using magnetic resonance imaging
title_short	The relationship between tissue oxygenation and redox status using magnetic resonance imaging
title_full	The relationship between tissue oxygenation and redox status using magnetic resonance imaging
title_fullStr	The relationship between tissue oxygenation and redox status using magnetic resonance imaging
title_full_unstemmed	The relationship between tissue oxygenation and redox status using magnetic resonance imaging
title_sort	relationship between tissue oxygenation and redox status using magnetic resonance imaging
description	The recent development of a bi-modality magnetic resonance imaging/electron paramagnetic resonance imaging (MRI/EPRI) platform has enabled longitudinal monitoring of both tumor oxygenation and redox status in murine cancer models. The current study used this imaging platform to test the hypothesis that a more reducing tumor microenvironment accompanies the development of tumor hypoxia. To test this, the redox status of the tumor was measured using Tempol as a redox-sensitive MRI contrast agent, and tumor hypoxia was measured with Oxo63, which is an oxygen-sensitive EPRI spin probe. Images were acquired every 1–2 days in mice bearing SCCVII tumors. The median pO2 decreased from 14 mmHg at 7 days after tumor implantation to 7 mmHg at 15 days after implantation. Additionally, the hypoxic fraction, defined as the percentage of the tumor that exhibited a pO2<10 mmHg, increased with tumor size (from 10% at 500 mm3 to 60% at 3,500 mm3). The rate of Tempol reduction increased as a function of tumor volume (0.4 min−1 at 500 mm3 to 1.7 min−1 at 3,500 mm3), suggesting that the tumor microenvironment became more reduced as the tumor grew. The results show that rapid Tempol reduction correlates with decreased tumor oxygenation, and that the Tempol decay rate constant may be a surrogate marker for tumor hypoxia.
publisher	D.A. Spandidos
publishDate	2012
url	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3583655/
_version_	1611957988995629056

The relationship between tissue oxygenation and redox status using magnetic resonance imaging

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